Resveratrol-induced changes of the human adipocyte secretion profile.
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Rosenow A, Noben JP, Jocken J, Kallendrusch S, Fischer-Posovszky P, Mariman EC, Renes J
Resveratrol-induced changes of the human adipocyte secretion profile.
J Proteome Res. 2012 Sep 7;11(9):4733-43. doi: 10.1021/pr300539b. Epub 2012 Aug 27.
- PubMed ID
- 22905912 [ View in PubMed]
- Abstract
Enlarged white adipose tissue (WAT) is a feature of obesity and leads to changes in its paracrine and endocrine function. Dysfunction of WAT cells is associated with obesity-associated disorders like type 2 diabetes and cardiovascular diseases. Resveratrol (RSV), a natural polyphenolic compound, mimics beneficial effects of calorie restriction. As such, RSV seems a promising therapeutic target for obesity-associated disorders. The effect of RSV on the human adipokine profile is still elusive. Therefore, a proteomic study together with bioinformatical analysis was performed to investigate the effect of RSV on the secretion profile of mature human SGBS adipocytes. RSV incubation resulted in elevated basal glycerol release and reduced intracellular TG content. This increased intracellular lipolysis was accompanied by profound changes in the adipocyte secretion profile. Extracellular matrix proteins were down-regulated while processing proteins were mostly up-regulated after RSV treatment. Interestingly, RSV induced secretion of proteins protective against cellular stress and proteins involved in the regulation of apoptosis. Furthermore, we found a RSV-induced up-regulation of adiponectin and ApoE accompanied by a down-regulation of PAI-1 and PEDF secretion which may improve anti-inflammatory processes and increased insulin sensitivity. These effects may contribute to alleviate obesity-induced metabolic complications. In addition, two novel RSV-regulated adipocyte-secreted proteins were identified.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Collagen alpha-1(I) chain P02452 Details Creatine kinase B-type P12277 Details Plasminogen activator inhibitor 1 P05121 Details L-lactate dehydrogenase A chain P00338 Details Aminoacylase-1 Q03154 Details Serotransferrin P02787 Details Glyceraldehyde-3-phosphate dehydrogenase P04406 Details Malate dehydrogenase, cytoplasmic P40925 Details 72 kDa type IV collagenase P08253 Details Dihydrolipoyl dehydrogenase, mitochondrial P09622 Details Endoplasmic reticulum chaperone BiP P11021 Details Apolipoprotein E P02649 Details Phosphatidylethanolamine-binding protein 1 P30086 Details Fibronectin P02751 Details Fatty acid-binding protein, adipocyte P15090 Details Fructose-bisphosphate aldolase A P04075 Details Complement factor D P00746 Details Peroxiredoxin-2 P32119 Details Laminin subunit gamma-1 P11047 Details Calreticulin P27797 Details Collagen alpha-1(III) chain P02461 Details Procathepsin L P07711 Details Collagen alpha-2(I) chain P08123 Details Superoxide dismutase [Cu-Zn] P00441 Details Nucleoside diphosphate kinase A P15531 Details Nucleoside diphosphate kinase B P22392 Details Triosephosphate isomerase P60174 Details Profilin-1 P07737 Details Vimentin P08670 Details Galectin-1 P09382 Details Peroxiredoxin-6 P30041 Details Dihydropyrimidinase-related protein 2 Q16555 Details Alpha-enolase P06733 Details Peroxiredoxin-1 Q06830 Details Nucleobindin-1 Q02818 Details 60 kDa heat shock protein, mitochondrial P10809 Details Heterogeneous nuclear ribonucleoproteins A2/B1 P22626 Details Protein disulfide-isomerase A3 P30101 Details Pigment epithelium-derived factor P36955 Details Haptoglobin P00738 Details