Resveratrol-induced changes of the human adipocyte secretion profile.

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Rosenow A, Noben JP, Jocken J, Kallendrusch S, Fischer-Posovszky P, Mariman EC, Renes J

Resveratrol-induced changes of the human adipocyte secretion profile.

J Proteome Res. 2012 Sep 7;11(9):4733-43. doi: 10.1021/pr300539b. Epub 2012 Aug 27.

PubMed ID
22905912 [ View in PubMed
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Abstract

Enlarged white adipose tissue (WAT) is a feature of obesity and leads to changes in its paracrine and endocrine function. Dysfunction of WAT cells is associated with obesity-associated disorders like type 2 diabetes and cardiovascular diseases. Resveratrol (RSV), a natural polyphenolic compound, mimics beneficial effects of calorie restriction. As such, RSV seems a promising therapeutic target for obesity-associated disorders. The effect of RSV on the human adipokine profile is still elusive. Therefore, a proteomic study together with bioinformatical analysis was performed to investigate the effect of RSV on the secretion profile of mature human SGBS adipocytes. RSV incubation resulted in elevated basal glycerol release and reduced intracellular TG content. This increased intracellular lipolysis was accompanied by profound changes in the adipocyte secretion profile. Extracellular matrix proteins were down-regulated while processing proteins were mostly up-regulated after RSV treatment. Interestingly, RSV induced secretion of proteins protective against cellular stress and proteins involved in the regulation of apoptosis. Furthermore, we found a RSV-induced up-regulation of adiponectin and ApoE accompanied by a down-regulation of PAI-1 and PEDF secretion which may improve anti-inflammatory processes and increased insulin sensitivity. These effects may contribute to alleviate obesity-induced metabolic complications. In addition, two novel RSV-regulated adipocyte-secreted proteins were identified.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Collagen alpha-1(I) chainP02452Details
Creatine kinase B-typeP12277Details
Plasminogen activator inhibitor 1P05121Details
L-lactate dehydrogenase A chainP00338Details
Aminoacylase-1Q03154Details
SerotransferrinP02787Details
Glyceraldehyde-3-phosphate dehydrogenaseP04406Details
Malate dehydrogenase, cytoplasmicP40925Details
72 kDa type IV collagenaseP08253Details
Dihydrolipoyl dehydrogenase, mitochondrialP09622Details
Endoplasmic reticulum chaperone BiPP11021Details
Apolipoprotein EP02649Details
Phosphatidylethanolamine-binding protein 1P30086Details
FibronectinP02751Details
Fatty acid-binding protein, adipocyteP15090Details
Fructose-bisphosphate aldolase AP04075Details
Complement factor DP00746Details
Peroxiredoxin-2P32119Details
Laminin subunit gamma-1P11047Details
CalreticulinP27797Details
Collagen alpha-1(III) chainP02461Details
Procathepsin LP07711Details
Collagen alpha-2(I) chainP08123Details
Superoxide dismutase [Cu-Zn]P00441Details
Nucleoside diphosphate kinase AP15531Details
Nucleoside diphosphate kinase BP22392Details
Triosephosphate isomeraseP60174Details
Profilin-1P07737Details
VimentinP08670Details
Galectin-1P09382Details
Peroxiredoxin-6P30041Details
Dihydropyrimidinase-related protein 2Q16555Details
Alpha-enolaseP06733Details
Peroxiredoxin-1Q06830Details
Nucleobindin-1Q02818Details
60 kDa heat shock protein, mitochondrialP10809Details
Heterogeneous nuclear ribonucleoproteins A2/B1P22626Details
Protein disulfide-isomerase A3P30101Details
Pigment epithelium-derived factorP36955Details
HaptoglobinP00738Details