The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.

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Murray J, Zhang B, Taylor SW, Oglesbee D, Fahy E, Marusich MF, Ghosh SS, Capaldi RA

The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.

J Biol Chem. 2003 Apr 18;278(16):13619-22. Epub 2003 Feb 28.

PubMed ID
12611891 [ View in PubMed
]
Abstract

Defects of the NADH dehydrogenase complex are predominantly manifested in mitochondrial diseases and are significantly associated with the development of many late onset neurological disorders such as Parkinson's disease. Here we describe an immunocapture procedure for isolating this multisubunit membrane-bound complex from human tissue. Using small amounts of immunoisolated protein, one-dimensional and two-dimensional gel electrophoresis, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) peptide mass finger printing (PMF), and nanoflow liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS), we can resolve and identify the human homologues of 42 polypeptides detected so far in the more extensively studied beef heart complex I. These polypeptides include the GRIM-19 protein, which is claimed to be involved in apoptosis, a polypeptide first identified by gene screening as a neuronal protein, as well as a protein thought to be in differentiation linked processes. The concordance of data from human and bovine complex I isolated by different procedures adds to the certainty that these novel proteins of seemingly diverse function are a part of complex I.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrialP56181Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialO00217Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrialO95299Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5O43920Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrialO75380Details
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialP19404Details
Cytochrome c oxidase subunit NDUFA4O00483Details
NADH dehydrogenase [ubiquinone] 1 subunit C2O95298Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrialO75489Details
NADH-ubiquinone oxidoreductase chain 3P03897Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6P56556Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1O15239Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrialO43674Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10O96000Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3O43676Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4O95168Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrialQ16795Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrialO75251Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1O75438Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8P51970Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6O95139Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7O95182Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrialO95178Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5Q16718Details
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialP49821Details
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialP28331Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrialO43181Details
NADH-ubiquinone oxidoreductase chain 1P03886Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7P17568Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13Q9P0J0Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11Q86Y39Details
Acyl carrier protein, mitochondrialO14561Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2O43678Details
NADH-ubiquinone oxidoreductase chain 4P03905Details
NADH-ubiquinone oxidoreductase chain 5P03915Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9Q9Y6M9Details
NADH-ubiquinone oxidoreductase chain 2P03891Details
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrialO95169Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3O95167Details
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialO75306Details
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12Q9UI09Details