PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.

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Jin P, Fu GK, Wilson AD, Yang J, Chien D, Hawkins PR, Au-Young J, Stuve LL

PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.

Genomics. 2004 Apr;83(4):566-71.

PubMed ID
15028279 [ View in PubMed
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Abstract

Alternative splicing of pre-mRNAs is an important mechanism for the generation of vertebrate protein diversity. Unfortunately, the contribution of alternative splicing to protein diversity is currently not well understood because many full-length mRNA sequences have yet to be identified. In this report, we describe the use of RT-PCR to identify and clone 279 novel alternatively spliced mRNAs from 114 well-known drug target genes. Our findings demonstrate the existence of many novel alternatively spliced mRNA transcripts and suggest that many more genes undergo functionally significant alternative splicing than previously thought.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Glycine receptor subunit betaP48167Details
Prolyl 4-hydroxylase subunit alpha-1P13674Details
5-hydroxytryptamine receptor 3AP46098Details
Sex hormone-binding globulinP04278Details
Inosine-5'-monophosphate dehydrogenase 1P20839Details
T-cell surface glycoprotein CD3 delta chainP04234Details