Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment.

Article Details


Carrascal M, Ovelleiro D, Casas V, Gay M, Abian J

Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment.

J Proteome Res. 2008 Dec;7(12):5167-76.

PubMed ID
19367720 [ View in PubMed

T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving preparative SDS-PAGE for prefractionation, in-gel digestion and sequential phosphopeptide enrichment using IMAC and TiO2. A total of 281 phosphorylation sites (197 of high confidence, Ascore > 15), mapping to 204 human gene sequences, were identified by LC-MS(n) analysis in an LTQ linear ion trap. Subsequently, we created the LymPHOS database (, which links mass spectrometric peptide information to phosphorylation sites and phosphoprotein sequences.

DrugBank Data that Cites this Article

NameUniProt ID
B-lymphocyte antigen CD20P11836Details
CD44 antigenP16070Details
Heat shock protein HSP 90-alphaP07900Details
Tubulin beta-3 chainQ13509Details
DNA topoisomerase 2-betaQ02880Details
Prostaglandin E synthase 3Q15185Details
cAMP-dependent protein kinase type I-alpha regulatory subunitP10644Details
Cation-independent mannose-6-phosphate receptorP11717Details
Triosephosphate isomeraseP60174Details
Proteasome subunit alpha type-3P25788Details
Membrane-associated progesterone receptor component 1O00264Details
Hepatoma-derived growth factorP51858Details
Protein S100-A9P06702Details
Tumor protein D54O43399Details
Ubiquitin carboxyl-terminal hydrolase 14P54578Details
Heterogeneous nuclear ribonucleoprotein D0Q14103Details
Heterochromatin protein 1-binding protein 3Q5SSJ5Details
AP2-associated protein kinase 1Q2M2I8Details
Serine/threonine-protein kinase 10O94804Details