Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment.

Article Details

Citation
Copy to clipboard

Carrascal M, Ovelleiro D, Casas V, Gay M, Abian J

Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment.

J Proteome Res. 2008 Dec;7(12):5167-76.

PubMed ID
19367720 [ View in PubMed
]
Abstract

T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving preparative SDS-PAGE for prefractionation, in-gel digestion and sequential phosphopeptide enrichment using IMAC and TiO2. A total of 281 phosphorylation sites (197 of high confidence, Ascore > 15), mapping to 204 human gene sequences, were identified by LC-MS(n) analysis in an LTQ linear ion trap. Subsequently, we created the LymPHOS database (http://lymphos.org), which links mass spectrometric peptide information to phosphorylation sites and phosphoprotein sequences.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
B-lymphocyte antigen CD20P11836Details
CD44 antigenP16070Details
Heat shock protein HSP 90-alphaP07900Details
Tubulin beta-3 chainQ13509Details
DNA topoisomerase 2-betaQ02880Details
Prostaglandin E synthase 3Q15185Details
cAMP-dependent protein kinase type I-alpha regulatory subunitP10644Details
Cation-independent mannose-6-phosphate receptorP11717Details
Triosephosphate isomeraseP60174Details
Proteasome subunit alpha type-3P25788Details
Epsin-1Q9Y6I3Details
Membrane-associated progesterone receptor component 1O00264Details
Hepatoma-derived growth factorP51858Details
Protein S100-A9P06702Details
Tumor protein D54O43399Details
Ubiquitin carboxyl-terminal hydrolase 14P54578Details
Filamin-AP21333Details
Heterogeneous nuclear ribonucleoprotein D0Q14103Details
Heterochromatin protein 1-binding protein 3Q5SSJ5Details
Myosin-9P35579Details
AP2-associated protein kinase 1Q2M2I8Details
Serine/threonine-protein kinase 10O94804Details