AP2-associated protein kinase 1

Details

Name

AP2-associated protein kinase 1

Synonyms

• 2.7.11.1
• Adaptor-associated kinase 1
• KIAA1048

Gene Name

AAK1

Organism

Humans

Amino acid sequence

>lcl|BSEQ0051696|AP2-associated protein kinase 1
MKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIV
FLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVW
EVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVE
NILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIIT
TKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDK
RPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTT
ETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKP
QAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPP
AQQQPAGTFYQQQQAQTQQFQAVHPATQKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQ
QQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPVRQQ
PKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAA
AEASLNKSKSATTTPSGSPRTSQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLG
EGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSDPFI
PLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPV
PQRLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTTDLLEEFAPTAISAPVHKAAEDSNL
ISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLID
L

Number of residues

961

Molecular Weight

103884.3

Theoretical pI

Not Available

GO Classification

Functions

AP-2 adaptor complex binding / ATP binding / Notch binding / protein serine/threonine kinase activity

Processes

endocytosis / membrane organization / positive regulation of Notch signaling pathway / protein autophosphorylation / protein phosphorylation / protein stabilization / regulation of clathrin-dependent endocytosis / regulation of protein localization

Components

cell leading edge / clathrin-coated pit / clathrin-coated vesicle / cytoplasm / cytosol / extrinsic component of plasma membrane / terminal bouton

General Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

Specific Function

Ap-2 adaptor complex binding

Pfam Domain Function

• Pkinase (PF00069)

Transmembrane Regions

Not Available

Cellular Location

Cell membrane

Gene sequence

>lcl|BSEQ0051697|AP2-associated protein kinase 1 (AAK1)
ATGAAGAAGTTTTTCGACTCCCGGCGAGAGCAGGGCGGCTCTGGCCTGGGCTCCGGCTCC
AGCGGAGGAGGGGGCAGCACCTCGGGCCTGGGCAGTGGCTACATCGGAAGAGTCTTCGGC
ATCGGGCGACAGCAGGTCACAGTGGACGAGGTGTTGGCGGAAGGTGGATTTGCTATTGTA
TTTCTGGTGAGGACAAGCAATGGGATGAAATGTGCCTTGAAACGCATGTTTGTCAACAAT
GAGCATGATCTCCAGGTGTGCAAGAGAGAAATCCAGATAATGAGGGATCTTTCAGGGCAC
AAGAATATTGTGGGTTACATTGATTCTAGTATCAACAACGTGAGTAGCGGTGATGTATGG
GAAGTGCTCATTCTGATGGACTTTTGTAGAGGTGGCCAGGTGGTAAACCTGATGAACCAG
CGCCTGCAAACAGGCTTTACAGAGAATGAAGTGCTCCAGATATTTTGTGATACCTGTGAA
GCTGTTGCCCGCCTGCATCAGTGCAAAACTCCTATTATCCACCGGGACCTGAAGGTTGAA
AACATCCTCTTGCATGACCGAGGCCACTATGTCCTGTGTGACTTTGGAAGCGCCACCAAC
AAATTCCAGAATCCACAAACTGAGGGAGTCAATGCAGTAGAAGATGAGATTAAGAAATAC
ACAACGCTGTCCTATCGAGCACCAGAAATGGTCAACCTGTACAGTGGCAAAATCATCACT
ACGAAGGCAGACATTTGGGCTCTTGGATGTTTGTTGTATAAATTATGCTACTTCACTTTG
CCATTTGGGGAAAGTCAGGTGGCAATTTGTGATGGAAACTTCACAATTCCTGATAATTCT
CGATATTCTCAAGACATGCACTGCCTAATTAGGTATATGTTGGAACCAGACCCTGACAAA
AGGCCGGATATTTACCAGGTGTCCTACTTCTCATTTAAGCTACTCAAGAAAGAGTGCCCA
ATTCCAAATGTACAGAACTCTCCCATTCCTGCAAAGCTTCCTGAACCAGTGAAAGCCAGT
GAGGCAGCTGCAAAAAAGACCCAGCCAAAGGCCAGACTGACAGATCCCATTCCCACCACA
GAGACTTCAATTGCACCCCGCCAGAGGCCTAAAGCTGGGCAGACTCAGCCGAACCCAGGA
ATCCTTCCCATCCAGCCAGCGCTGACACCCCGGAAGAGGGCCACTGTTCAGCCCCCACCT
CAGGCTGCAGGATCCAGCAATCAGCCTGGCCTTTTAGCCAGTGTTCCCCAACCAAAACCC
CAAGCCCCACCCAGCCAGCCTCTGCCGCAAACTCAGGCCAAGCAGCCACAGGCTCCTCCC
ACTCCACAGCAGACGCCTTCTACTCAGGCCCAGGGTCTGCCCGCTCAGGCCCAGGCCACA
CCCCAGCACCAGCAGCAACTCTTCCTCAAGCAGCAACAGCAGCAGCAACAGCCACCGCCA
GCACAGCAGCAGCCGGCAGGCACGTTTTACCAGCAGCAGCAGGCCCAGACTCAGCAGTTT
CAGGCAGTACATCCAGCAACCCAGCAACCAGCAATTGCTCAGTTCCCTGTGGTGTCCCAA
GGAGGCTCTCAACAGCAGCTAATGCAGAATTTCTACCAGCAGCAGCAGCAGCAGCAACAA
CAACAGCAACAGCAACAGCTGGCCACAGCCCTGCATCAACAACAGCTGATGACTCAGCAG
GCTGCCTTGCAGCAAAAGCCCACTATGGCAGCAGGACAGCAGCCCCAGCCACAGCCAGCT
GCAGCCCCACAGCCAGCCCCTGCCCAGGAGCCAGCGATTCAAGCCCCAGTAAGACAACAG
CCAAAGGTTCAGACAACCCCACCTCCTGCCGTCCAGGGGCAGAAAGTTGGATCTCTCACT
CCACCCTCATCCCCCAAAACCCAACGTGCTGGGCACAGGCGTATTCTCAGTGACGTAACC
CACAGTGCAGTCTTTGGGGTCCCTGCCAGCAAATCAACCCAGCTGCTCCAGGCAGCTGCA
GCTGAGGCCAGTCTCAATAAGTCCAAGTCTGCAACCACCACTCCATCAGGCTCTCCTCGG
ACCTCTCAACAAAACGTTTATAATCCTTCAGAAGGGTCTACGTGGAATCCCTTTGATGAC
GATAATTTCTCCAAACTCACAGCTGAAGAACTGCTAAACAAGGACTTTGCCAAGCTTGGG
GAAGGCAAACATCCCGAGAAGCTTGGAGGCTCAGCTGAGAGTTTGATCCCAGGCTTTCAA
TCAACCCAAGGTGATGCTTTTGCTACGACCTCATTTTCTGCTGGAACTGCTGAAAAAAGG
AAGGGTGGGCAGACTGTGGACTCTGGCCTCCCGCTTCTAAGCGTGTCTGATCCTTTCATT
CCTCTTCAAGTACCTGATGCACCAGAAAAACTAATTGAGGGACTCAAATCTCCTGACACT
TCTCTTCTGCTCCCTGACCTCTTGCCTATGACAGATCCTTTTGGTAGCACTTCTGATGCT
GTAATTGAAAAAGCTGATGTTGCTGTTGAGAGTCTCATACCAGGACTGGAGCCCCCAGTT
CCCCAGCGCCTCCCATCTCAGACGGAATCTGTGACCTCGAATCGCACAGATTCTCTCACC
GGGGAAGATTCCCTGCTTGATTGCTCTCTGCTCTCTAACCCTACTACTGACCTTCTGGAA
GAGTTTGCCCCCACAGCAATCTCTGCTCCAGTCCATAAAGCTGCAGAAGATAGTAATCTC
ATCTCAGGTTTTGATGTCCCTGAGGGCTCGGACAAGGTGGCTGAAGATGAGTTTGACCCT
ATTCCTGTATTGATAACCAAAAACCCACAAGGTGGGCACTCTAGAAACAGCAGTGGGAGC
TCTGAGTCCAGTCTTCCCAACCTAGCCAGGTCTTTACTGCTGGTGGATCAGCTCATAGAC
CTGTAG

Chromosome Location

2

Locus

2p13.3

External Identifiers

Resource	Link
UniProtKB ID	Q2M2I8
UniProtKB Entry Name	AAK1_HUMAN
HGNC ID	HGNC:19679

General References

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Drug Relations

Drug Relations

DrugBank ID	Name	Drug group	Pharmacological action?	Actions	Details
DB12010	Fostamatinib	approved, investigational	unknown	inhibitor	Details