Orotate phosphoribosyltransferase

Details

Name
Orotate phosphoribosyltransferase
Synonyms
  • 2.4.2.10
  • OPRT
Gene Name
pyrE
UniProtKB Entry
P08870Swiss-Prot
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
NCBI Taxonomy ID
99287
Amino acid sequence
>lcl|BSEQ0011085|Orotate phosphoribosyltransferase
MKPYQRQFIEFALNKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLALLGRFYAEALVDS
GIEFDLLFGPAYKGIPIATTTAVALAEHHDKDLPYCFNRKEAKDHGEGGSLVGSALQGRV
MLVDDVITAGTAIRESMEIIQAHGATLAGVLISLDRQERGRGEISAIQEVERDYGCKVIS
IITLKDLIAYLEEKPDMAEHLAAVRAYREEFGV
Number of residues
213
Molecular Weight
23561.74
Theoretical pI
5.68
GO Classification
Functions
magnesium ion binding / orotate phosphoribosyltransferase activity
Processes
'de novo' UMP biosynthetic process
General Function
Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
Specific Function
magnesium ion binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>lcl|BSEQ0011086|Orotate phosphoribosyltransferase (pyrE)
ATGAAACCGTATCAGCGCCAGTTTATTGAGTTTGCGCTTAACAAGCAGGTACTAAAGTTT
GGCGAGTTTACGCTGAAATCCGGGCGCAAAAGCCCCTATTTCTTCAACGCCGGGCTGTTT
AATACCGGGCGCGACCTGGCGTTGTTAGGCCGTTTTTATGCCGAAGCGCTGGTGGATTCC
GGTATTGAATTTGATCTGCTGTTTGGCCCCGCGTACAAAGGTATTCCGATCGCGACGACG
ACGGCGGTTGCGCTGGCGGAACATCACGATAAAGACCTGCCGTACTGCTTTAACCGCAAA
GAGGCAAAAGATCATGGTGAAGGCGGGAGCCTGGTGGGCAGCGCGCTACAGGGCCGCGTG
ATGCTGGTGGATGACGTGATTACCGCAGGTACGGCGATTCGCGAGTCAATGGAGATTATT
CAGGCGCACGGGGCTACGCTGGCTGGCGTACTGATTTCGCTGGATCGTCAGGAACGTGGC
CGCGGCGAGATTTCCGCGATTCAGGAAGTGGAGCGCGACTATGGTTGTAAGGTTATCTCC
ATCATTACGCTGAAAGACCTGATTGCCTATCTGGAAGAAAAACCTGATATGGCTGAGCAT
CTGGCGGCTGTGCGAGCATACCGGGAAGAATTTGGCGTGTAA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP08870
UniProtKB Entry NamePYRE_SALTY
GenBank Protein ID47812
GenBank Gene IDZ19547
PDB ID(s)1LH0, 1OPR, 1STO
KEGG IDstm:STM3733
NCBI Gene ID1255257
General References
  1. Scapin G, Sacchettini JC, Dessen A, Bhatia M, Grubmeyer C: Primary structure and crystallization of orotate phosphoribosyltransferase from Salmonella typhimurium. J Mol Biol. 1993 Apr 20;230(4):1304-8. [Article]
  2. McClelland M, Sanderson KE, Spieth J, Clifton SW, Latreille P, Courtney L, Porwollik S, Ali J, Dante M, Du F, Hou S, Layman D, Leonard S, Nguyen C, Scott K, Holmes A, Grewal N, Mulvaney E, Ryan E, Sun H, Florea L, Miller W, Stoneking T, Nhan M, Waterston R, Wilson RK: Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature. 2001 Oct 25;413(6858):852-6. [Article]
  3. Neuhard J, Stauning E, Kelln RA: Cloning and characterization of the pyrE gene and of PyrE::Mud1 (Ap lac) fusions from Salmonella typhimurium. Eur J Biochem. 1985 Feb 1;146(3):597-603. [Article]
  4. Bhatia MB, Vinitsky A, Grubmeyer C: Kinetic mechanism of orotate phosphoribosyltransferase from Salmonella typhimurium. Biochemistry. 1990 Nov 20;29(46):10480-7. [Article]
  5. Bhatia MB, Grubmeyer C: The role of divalent magnesium in activating the reaction catalyzed by orotate phosphoribosyltransferase. Arch Biochem Biophys. 1993 Jun;303(2):321-5. [Article]
  6. Grubmeyer C, Segura E, Dorfman R: Active site lysines in orotate phosphoribosyltransferase. J Biol Chem. 1993 Sep 25;268(27):20299-304. [Article]
  7. Ozturk DH, Dorfman RH, Scapin G, Sacchettini JC, Grubmeyer C: Locations and functional roles of conserved lysine residues in Salmonella typhimurium orotate phosphoribosyltransferase. Biochemistry. 1995 Aug 29;34(34):10755-63. [Article]
  8. Ozturk DH, Dorfman RH, Scapin G, Sacchettini JC, Grubmeyer C: Structure and function of Salmonella typhimurium orotate phosphoribosyltransferase: protein complementation reveals shared active sites. Biochemistry. 1995 Aug 29;34(34):10764-70. [Article]
  9. Scapin G, Grubmeyer C, Sacchettini JC: Crystal structure of orotate phosphoribosyltransferase. Biochemistry. 1994 Feb 15;33(6):1287-94. [Article]
  10. Scapin G, Ozturk DH, Grubmeyer C, Sacchettini JC: The crystal structure of the orotate phosphoribosyltransferase complexed with orotate and alpha-D-5-phosphoribosyl-1-pyrophosphate. Biochemistry. 1995 Aug 29;34(34):10744-54. [Article]

Associated Data

Bio-Entities
Bio-EntityType
Orotate phosphoribosyltransferase (Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720))protein
primary
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
5-O-phosphono-alpha-D-ribofuranosyl diphosphateapproved, experimental, investigationalunknowntargetsubstrateDetails
Orotic acidinvestigationalunknowntargetDetails