Glycerol uptake facilitator protein
Details
- Name
- Glycerol uptake facilitator protein
- Synonyms
- Aquaglyceroporin
- Gene Name
- glpF
- Organism
- Escherichia coli (strain K12)
- Amino acid sequence
>lcl|BSEQ0016653|Glycerol uptake facilitator protein MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISVIWGLGVAMAIYLTA GVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAALVYGLYYNLFFDFEQTHH IVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILMGLILALTDDGNGVPRGPLAP LLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAGWGNVAFTGGRDIPYFLVPLFGP IVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSEQKASL
- Number of residues
- 281
- Molecular Weight
- 29779.71
- Theoretical pI
- 6.66
- GO Classification
- Functionsglycerol channel activity / glycerol transmembrane transporter activity / metal ion binding / water channel activityProcessescellular response to mercury ion / cellular water homeostasis / glycerol transport / ion transmembrane transport / water transportComponentsintegral component of plasma membrane / plasma membrane
- General Function
- Water channel activity
- Specific Function
- Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
- Pfam Domain Function
- MIP (PF00230)
- Transmembrane Regions
- 6-34 40-60 85-108 144-169 178-194 232-254
- Cellular Location
- Cell inner membrane
- Gene sequence
>lcl|BSEQ0016654|Glycerol uptake facilitator protein (glpF) ATGAGTCAAACATCAACCTTGAAAGGCCAGTGCATTGCTGAATTCCTCGGTACCGGGTTG TTGATTTTCTTCGGTGTGGGTTGCGTTGCAGCACTAAAAGTCGCTGGTGCGTCTTTTGGT CAGTGGGAAATCAGTGTCATTTGGGGACTGGGGGTGGCAATGGCCATCTACCTGACCGCA GGGGTTTCCGGCGCGCATCTTAATCCCGCTGTTACCATTGCATTGTGGCTGTTTGCCTGT TTCGACAAGCGCAAAGTTATTCCTTTTATCGTTTCACAAGTTGCCGGCGCTTTCTGTGCT GCGGCTTTAGTTTACGGGCTTTACTACAATTTATTTTTCGACTTCGAGCAGACTCATCAC ATTGTTCGCGGCAGCGTTGAAAGTGTTGATCTGGCTGGCACTTTCTCTACTTACCCTAAT CCTCATATCAATTTTGTGCAGGCTTTCGCAGTTGAGATGGTGATTACCGCTATTCTGATG GGGCTGATCCTGGCGTTAACGGACGATGGCAACGGTGTACCACGCGGCCCTTTGGCTCCC TTGCTGATTGGTCTACTGATTGCGGTCATTGGCGCATCTATGGGCCCATTGACAGGTTTT GCCATGAACCCAGCGCGTGACTTCGGTCCGAAAGTCTTTGCCTGGCTGGCGGGCTGGGGC AATGTCGCCTTTACCGGCGGCAGAGACATTCCTTACTTCCTGGTGCCGCTTTTCGGCCCT ATCGTTGGCGCGATTGTAGGTGCATTTGCCTACCGCAAACTGATTGGTCGCCATTTGCCT TGCGATATCTGTGTTGTGGAAGAAAAGGAAACCACAACTCCTTCAGAACAAAAAGCTTCG CTGTAA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID P0AER0 UniProtKB Entry Name GLPF_ECOLI GenBank Gene ID X15054 - General References
- Muramatsu S, Mizuno T: Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli. Nucleic Acids Res. 1989 Jun 12;17(11):4378. [Article]
- Weissenborn DL, Wittekindt N, Larson TJ: Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12. J Biol Chem. 1992 Mar 25;267(9):6122-31. [Article]
- Plunkett G 3rd, Burland V, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 1993 Jul 25;21(15):3391-8. [Article]
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- Borgnia MJ, Agre P: Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2888-93. Epub 2001 Feb 20. [Article]
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- Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, Krucinski J, Stroud RM: Structure of a glycerol-conducting channel and the basis for its selectivity. Science. 2000 Oct 20;290(5491):481-6. [Article]
- Unger VM: Fraternal twins: AQP1 and GlpF. Nat Struct Biol. 2000 Dec;7(12):1082-4. [Article]
- Tajkhorshid E, Nollert P, Jensen MO, Miercke LJ, O'Connell J, Stroud RM, Schulten K: Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science. 2002 Apr 19;296(5567):525-30. [Article]