Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-alpha-acetylation features.

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Bienvenut WV, Sumpton D, Martinez A, Lilla S, Espagne C, Meinnel T, Giglione C

Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-alpha-acetylation features.

Mol Cell Proteomics. 2012 Jun;11(6):M111.015131. doi: 10.1074/mcp.M111.015131. Epub 2012 Jan 5.

PubMed ID
22223895 [ View in PubMed
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Abstract

N-terminal modifications play a major role in the fate of proteins in terms of activity, stability, or subcellular compartmentalization. Such modifications remain poorly described and badly characterized in proteomic studies, and only a few comparison studies among organisms have been made available so far. Recent advances in the field now allow the enrichment and selection of N-terminal peptides in the course of proteome-wide mass spectrometry analyses. These targeted approaches unravel as a result the extent and nature of the protein N-terminal modifications. Here, we aimed at studying such modifications in the model plant Arabidopsis thaliana to compare these results with those obtained from a human sample analyzed in parallel. We applied large scale analysis to compile robust conclusions on both data sets. Our data show strong convergence of the characterized modifications especially for protein N-terminal methionine excision, co-translational N-alpha-acetylation, or N-myristoylation between animal and plant kingdoms. Because of the convergence of both the substrates and the N-alpha-acetylation machinery, it was possible to identify the N-acetyltransferases involved in such modifications for a small number of model plants. Finally, a high proportion of nuclear-encoded chloroplast proteins feature post-translational N-alpha-acetylation of the mature protein after removal of the transit peptide. Unlike animals, plants feature in a dedicated pathway for post-translational acetylation of organelle-targeted proteins. The corresponding machinery is yet to be discovered.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Phenylalanine--tRNA ligase alpha subunitQ9Y285Details
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingQ15738Details
Adenine phosphoribosyltransferaseP07741Details
Alpha-aminoadipic semialdehyde dehydrogenaseP49419Details
Monocarboxylate transporter 8P36021Details
Glutathione synthetaseP48637Details
Lysine--tRNA ligaseQ15046Details
Valine--tRNA ligaseP26640Details
Tryptophan--tRNA ligase, cytoplasmicP23381Details
Choline/ethanolamine kinaseQ9Y259Details
Purine nucleoside phosphorylaseP00491Details
Solute carrier family 12 member 2P55011Details
4-trimethylaminobutyraldehyde dehydrogenaseP49189Details
Alanine--tRNA ligase, cytoplasmicP49588Details
Spermidine synthaseP19623Details
CalmodulinP0DP23Details
L-lactate dehydrogenase A chainP00338Details
Aspartate aminotransferase, cytoplasmicP17174Details
Dihydropteridine reductaseP09417Details
Retinal dehydrogenase 1P00352Details
Guanidinoacetate N-methyltransferaseQ14353Details
C-1-tetrahydrofolate synthase, cytoplasmicP11586Details
ADP/ATP translocase 3P12236Details
ADP/ATP translocase 2P05141Details
Farnesyl pyrophosphate synthaseP14324Details
Glutathione S-transferase PP09211Details
Perilipin-3O60664Details
L-xylulose reductaseQ7Z4W1Details
Mitogen-activated protein kinase 1P28482Details
Fatty acid synthaseP49327Details
Peptidyl-prolyl cis-trans isomerase AP62937Details
Choline transporter-like protein 1Q8WWI5Details
Mitogen-activated protein kinase 3P27361Details
Glucose-6-phosphate 1-dehydrogenaseP11413Details
Caspase-3P42574Details
RAC-beta serine/threonine-protein kinaseP31751Details
14-3-3 protein zeta/deltaP63104Details
Fatty acid-binding protein, adipocyteP15090Details
Fatty acid-binding protein, heartP05413Details
m7GpppX diphosphataseQ96C86Details
Bifunctional purine biosynthesis protein PURHP31939Details
Thymidine kinase, cytosolicP04183Details
Acetyl-CoA acetyltransferase, cytosolicQ9BWD1Details
Uroporphyrinogen decarboxylaseP06132Details
Leukotriene A-4 hydrolaseP09960Details
Fructose-bisphosphate aldolase AP04075Details
Caspase-7P55210Details
Macrophage migration inhibitory factorP14174Details
Thioredoxin reductase 1, cytoplasmicQ16881Details
Fatty acid-binding protein, epidermalQ01469Details
Serpin B6P35237Details
Serine dehydratase-likeQ96GA7Details
Multifunctional protein ADE2P22234Details
CAD proteinP27708Details
Glutamine--tRNA ligaseP47897Details
N-acetyl-D-glucosamine kinaseQ9UJ70Details
Nicotinamide phosphoribosyltransferaseP43490Details
NAD-dependent protein deacetylase sirtuin-1Q96EB6Details
Voltage-dependent anion-selective channel protein 1P21796Details
Hypoxia-inducible factor 1-alpha inhibitorQ9NWT6Details
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1O43252Details
Calpain-1 catalytic subunitP07384Details
cAMP-dependent protein kinase type II-alpha regulatory subunitP13861Details
cAMP-dependent protein kinase type I-alpha regulatory subunitP10644Details
Signal transducer and activator of transcription 3P40763Details
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformP30153Details
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoformP63151Details
ADP-ribosylation factor 1P84077Details
60S ribosomal protein L13aP40429Details
60S ribosomal protein L11P62913Details
Proteasome subunit alpha type-3P25788Details
Proteasome subunit beta type-3P49720Details
Proteasome subunit beta type-4P28070Details
Platelet-activating factor acetylhydrolase IB subunit gammaQ15102Details
Profilin-1P07737Details
Calpain small subunit 1P04632Details
DestrinP60981Details
Cofilin-1P23528Details
Elongation factor 2P13639Details
Profilin-2P35080Details
Acyl-coenzyme A thioesterase 13Q9NPJ3Details
Mothers against decapentaplegic homolog 2Q15796Details
Protein arginine N-methyltransferase 3O60678Details
Galectin-1P09382Details
Glycogenin-1P46976Details
Signal recognition particle 14 kDa proteinP37108Details
Poly(A) polymerase alphaP51003Details
V-type proton ATPase subunit C 1P21283Details
GTP-binding nuclear protein RanP62826Details
Transitional endoplasmic reticulum ATPaseP55072Details
Spectrin beta chain, non-erythrocytic 1Q01082Details
Spliceosome RNA helicase DDX39BQ13838Details
Peroxiredoxin-6P30041Details
Breakpoint cluster region proteinP11274Details
Serine-protein kinase ATMQ13315Details
Actin, cytoplasmic 1P60709Details
Actin, cytoplasmic 2P63261Details
Actin, aortic smooth muscleP62736Details
Histone H1.4P10412Details
Chloride intracellular channel protein 1O00299Details
Programmed cell death protein 6O75340Details
Cytochrome c oxidase copper chaperoneQ14061Details
Calmodulin-2P0DP24Details
Calmodulin-3P0DP25Details
Protein S100-A8P05109Details
Heterogeneous nuclear ribonucleoprotein HP31943Details
Receptor of activated protein C kinase 1P63244Details
Stress-induced-phosphoprotein 1P31948Details
Serpin B9P50453Details
Cystatin-BP04080Details
Splicing factor 3B subunit 3Q15393Details
Heterogeneous nuclear ribonucleoprotein FP52597Details
Heat shock protein 105 kDaQ92598Details
Splicing factor 3A subunit 3Q12874Details
Tumor protein D54O43399Details
Tropomyosin alpha-4 chainP67936Details
Junction plakoglobinP14923Details
Far upstream element-binding protein 2Q92945Details
Ras GTPase-activating-like protein IQGAP1P46940Details
NucleophosminP06748Details
40S ribosomal protein S18P62269Details
40S ribosomal protein S5P46782Details
Splicing factor 1Q15637Details
Survival motor neuron proteinB4DP61Details
Interferon-induced, double-stranded RNA-activated protein kinaseP19525Details
Mitogen-activated protein kinase kinase kinase kinase 3Q8IVH8Details
Serine/threonine-protein kinase Nek9Q8TD19Details
Serine/threonine-protein kinase OSR1O95747Details
Serine/threonine-protein kinase PAK 2Q13177Details
Phosphatidylinositol 5-phosphate 4-kinase type-2 betaP78356Details
Serine/threonine-protein kinase PRP4 homologQ13523Details
Thiamine-triphosphataseQ9BU02Details
Elongation of very long chain fatty acids protein 1Q9BW60Details
Tyrosine-protein phosphatase non-receptor type 12Q05209Details
UbiA prenyltransferase domain-containing protein 1Q9Y5Z9Details
Signal transducer and activator of transcription 1-alpha/betaP42224Details
Histone acetyltransferase p300Q09472Details