Monoamine neurotransmitter transport mediated by the polyspecific cation transporter rOCT1.
Article Details
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Busch AE, Quester S, Ulzheimer JC, Gorboulev V, Akhoundova A, Waldegger S, Lang F, Koepsell H
Monoamine neurotransmitter transport mediated by the polyspecific cation transporter rOCT1.
FEBS Lett. 1996 Oct 21;395(2-3):153-6.
- PubMed ID
- 8898084 [ View in PubMed]
- Abstract
The polyspecific cation transporter rOCT,1 which is localized in the basolateral membrane of rat renal proximal tubules and in sinusoidal membranes of hepatocytes, was analyzed for transport of monoamine neurotransmitters. In voltage-clamp experiments with rOCT1-expressing Xenopus oocytes, superfusion with dopamine, serotonin, noradrenaline, histamine and the permanent cation acetylcholine induced saturable inwardly directed currents with apparent Km values ranging from 20 to 100 microM. Transport of dopamine was also demonstrated by uptake measurements in oocytes and in the mammalian cell line (HEK 293) which was permanently transfected with rOCT1. The high uptake rates measured in rOCT1-expressing oocytes and in transfected HEK 293 cells suggest that rOCT1 is a high capacity transporter which mediates the first step in the excretion of monoamine neurotransmitters.
DrugBank Data that Cites this Article
- Drug Transporters
Drug Transporter Kind Organism Pharmacological Action Actions Acetylcholine Solute carrier family 22 member 1 Protein Humans UnknownSubstrateDetails Dopamine Solute carrier family 22 member 1 Protein Humans UnknownSubstrateDetails Histamine Solute carrier family 22 member 1 Protein Humans UnknownNot Available Details Norepinephrine Solute carrier family 22 member 1 Protein Humans UnknownSubstrateDetails Quinidine Solute carrier family 22 member 1 Protein Humans UnknownInhibitorDetails Quinine Solute carrier family 22 member 1 Protein Humans UnknownSubstrateInhibitorDetails