Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.

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Impens F, Radoshevich L, Cossart P, Ribet D

Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.

Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12432-7. doi: 10.1073/pnas.1413825111. Epub 2014 Aug 11.

PubMed ID
25114211 [ View in PubMed
]
Abstract

SUMOylation is an essential ubiquitin-like modification involved in important biological processes in eukaryotic cells. Identification of small ubiquitin-related modifier (SUMO)-conjugated residues in proteins is critical for understanding the role of SUMOylation but remains experimentally challenging. We have set up a powerful and high-throughput method combining quantitative proteomics and peptide immunocapture to map SUMOylation sites and have analyzed changes in SUMOylation in response to stimuli. With this technique we identified 295 SUMO1 and 167 SUMO2 sites on endogenous substrates of human cells. We further used this strategy to characterize changes in SUMOylation induced by listeriolysin O, a bacterial toxin that impairs the host cell SUMOylation machinery, and identified several classes of host proteins specifically deSUMOylated in response to this toxin. Our approach constitutes an unprecedented tool, broadly applicable to various SUMO-regulated cellular processes in health and disease.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
40S ribosomal protein S2P15880Details
Stress-induced-phosphoprotein 1P31948Details
Hepatoma-derived growth factorP51858Details
Ran-specific GTPase-activating proteinP43487Details
Heterogeneous nuclear ribonucleoprotein KP61978Details
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1Q969G3Details
Proliferating cell nuclear antigenP12004Details
Far upstream element-binding protein 2Q92945Details
Filamin-AP21333Details
Heterogeneous nuclear ribonucleoprotein D0Q14103Details
NucleophosminP06748Details
60S ribosomal protein L4P36578Details
40S ribosomal protein S17P08708Details
Probable ATP-dependent RNA helicase DDX5P17844Details
Splicing factor 3B subunit 1O75533Details
Serine/threonine-protein kinase PRP4 homologQ13523Details
Signal transducer and activator of transcription 1-alpha/betaP42224Details
Proliferation marker protein Ki-67P46013Details