Effect of metal ions and calcium on purified PON1 and PON3 from rat liver.
Article Details
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Pla A, Rodrigo L, Hernandez AF, Gil F, Lopez O
Effect of metal ions and calcium on purified PON1 and PON3 from rat liver.
Chem Biol Interact. 2007 Apr 5;167(1):63-70. Epub 2007 Jan 16.
- PubMed ID
- 17292339 [ View in PubMed]
- Abstract
The effect of several metal ions and calcium on purified paraoxonases (PON1 and PON3) from rat liver was studied. PON1 and PON3 were also inhibited by EDTA and both enzyme activities were restored by the addition of free calcium. The reactivation by calcium was a time-dependent effect for PON1; however, this was not the case for PON3. We also studied the response of PON1 and PON3 to several inhibitors: Co, Cu, Mn, Hg and p-hydroxymercurybenzoate (pOHMB), and determined the type of inhibition and the inhibition constants. Among all the compounds tested, mercurials (Hg and pOHMB) were the most potent inhibitors of PON1. For PON3 mercurials and copper showed the highest inhibitory potency. Purified PON3 also showed different inhibition patterns as compared to PON1. A comparison of PON1 and PON3 shows qualitative and quantitative differences in the sensitivity against the inhibitors tested, showing major differences in the case of cobalt, copper and pOHMB, which may be related to structural differences of both PONs. These results increase our knowledge of the biochemical properties of PON1 and PON3 and may help in the understanding of their physiological role as a potential detoxification mechanism against environmental metal ions.
DrugBank Data that Cites this Article
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions Edetate calcium disodium anhydrous Serum paraoxonase/arylesterase 1 Protein Humans UnknownInhibitorDetails Edetate calcium disodium anhydrous Serum paraoxonase/lactonase 3 Protein Humans UnknownInhibitorDetails Edetate disodium anhydrous Serum paraoxonase/arylesterase 1 Protein Humans UnknownInhibitorDetails Edetate disodium anhydrous Serum paraoxonase/lactonase 3 Protein Humans UnknownInhibitorDetails Edetic acid Serum paraoxonase/lactonase 3 Protein Humans UnknownInhibitorDetails