Identification of new human CYP2C19 alleles (CYP2C19*6 and CYP2C19*2B) in a Caucasian poor metabolizer of mephenytoin.
Article Details
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Ibeanu GC, Goldstein JA, Meyer U, Benhamou S, Bouchardy C, Dayer P, Ghanayem BI, Blaisdell J
Identification of new human CYP2C19 alleles (CYP2C19*6 and CYP2C19*2B) in a Caucasian poor metabolizer of mephenytoin.
J Pharmacol Exp Ther. 1998 Sep;286(3):1490-5.
- PubMed ID
- 9732415 [ View in PubMed]
- Abstract
A genetic polymorphism in the metabolism of the anticonvulsant drug S-mephenytoin has been attributed to defective CYP2C19 alleles. This genetic polymorphism displays large interracial differences with the poor metabolizer (PM) phenotype representing 2-5% of Caucasian and 13-23% of Oriental populations. In the present study, we identified two new mutations in CYP2C19 in a single Swiss Caucasian PM outlier (JOB 1) whose apparent genotype (CYP2C19*1/CYP2C19*2) did not agree with his PM phenotype. These mutations consisted of a single base pair mutation (G395A) in exon 3 resulting in an Arg132-->Gln coding change and a (G276C) mutation in exon 2 resulting in a coding change Glu92-->Asp. However, the G276C mutation and the G395A mutation resided on separate alleles. Genotyping tests of a family study of JOB1 showed that the exon 2 change occurred on the CYP2C19*2 allele, which also contained the known splice mutation in exon 5 (this variant is termed CYP2C19*2B to distinguish it from the original splice variant now termed CYP2C19*2A). The exon 3 mutation resided on a separate allele (termed CYP2C19*6). In all other respects this allele was identical to one of two wild-type alleles, CYP2C19*1B. The incidence of CYP2C19*6 in a European Caucasian population phenotyped for mephenytoin metabolism was 0/344 (99% confidence limits of 0 to 0.9%). Seven of 46 Caucasian CYP2C19*2 alleles were CYP2C19*2B(15%) and 85% were CYP2C19*2A. The Arg132Gln mutation was produced by site-directed mutatgenesis and the recombinant protein expressed in a bacterial cDNA expression system. Recombinant CYP2C19 6 had negligible catalytic activity toward S-mephenytoin compared with CYP2C19 1B, which is consistent with the conclusion that CYP2C19*6 represents a PM allele. Thus, the new CYP2C19*6 allele contributes to the PM phenotype in Caucasians.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Cytochrome P450 2C19 P33261 Details - Pharmaco-genomics
Drug Interacting Gene/Enzyme Allele name Genotypes Defining change(s) Type(s) Description Details Amitriptyline Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
ADR Inferred Those with the AA or AG genotype are poor metabolizers of amitriptyline Details Amitriptyline Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
ADR Inferred Those with the AA or AG genotype are poor metabolizers of amitriptyline Details Omeprazole Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor metabolizer, lower dose requirement, improved drug efficacy Details Omeprazole Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor metabolizer, lower dose requirement, improved drug efficacy Details Clobazam Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
ADR Inferred Clobazem is metabolized into N-desmethylclobazem (NCLB) mostly by CYP3A4. NCLB is primarily metabolized by 2C19. Those with one 2C19*2 allele mutation (1*/2*) are intermediate metabolizers of NCLB. Those with two (2*/2*) mutations will metabolize NCLB poorly in comparisone to extensive metabolizers (1*/1*). Levels of NCLB can be five times higher in poor metabolizers, and two times higher in intermediate metabolizers as compared to individuals who are extensive metabolizers. The safety and efficacy of clobazem may be affected by polymorphic expression of CYP2C19*2. Details Clobazam Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
ADR Inferred Clobazem is metabolized into N-desmethylclobazem (NCLB) mostly by CYP3A4. NCLB is primarily metabolized by 2C19. Those with one 2C19*2 allele mutation (1*/2*) are intermediate metabolizers of NCLB. Those with two (2*/2*) mutations will metabolize NCLB poorly in comparisone to extensive metabolizers (1*/1*). Levels of NCLB can be five times higher in poor metabolizers, and two times higher in intermediate metabolizers as compared to individuals who are extensive metabolizers. The safety and efficacy of clobazem may be affected by polymorphic expression of CYP2C19*2. Details Lansoprazole Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor metabolizer, lower dose requirement, improved drug efficacy Details Lansoprazole Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor metabolizer, lower dose requirement, improved drug efficacy Details Mephenytoin Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor metabolizer, lower dose requirement Details Mephenytoin Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor metabolizer, lower dose requirement Details Esomeprazole Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor metabolizer, lower dose requirement, improved drug efficacy Details Esomeprazole Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor metabolizer, lower dose requirement, improved drug efficacy Details Clopidogrel Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor drug metabolizer, lower dose requirements, higher risk for drug-drug interactions Details Clopidogrel Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor drug metabolizer, lower dose requirements, higher risk for drug-drug interactions Details Proguanil Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor metabolizer, lower dose requirement Details Proguanil Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor metabolizer, lower dose requirement Details Gliclazide Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*2B Not Available - 681G>A (rs4244285)
Effect Inferred Poor drug metabolizer. Details Gliclazide Cytochrome P450 2C19
Gene symbol: CYP2C19
UniProt: P33261CYP2C19*6 Not Available - 395G>A (rs72552267)
Effect Inferred Poor drug metabolizer. Details