Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157.

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Jin Q, Yuan Z, Xu J, Wang Y, Shen Y, Lu W, Wang J, Liu H, Yang J, Yang F, Zhang X, Zhang J, Yang G, Wu H, Qu D, Dong J, Sun L, Xue Y, Zhao A, Gao Y, Zhu J, Kan B, Ding K, Chen S, Cheng H, Yao Z, He B, Chen R, Ma D, Qiang B, Wen Y, Hou Y, Yu J

Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157.

Nucleic Acids Res. 2002 Oct 15;30(20):4432-41.

PubMed ID
12384590 [ View in PubMed
]
Abstract

We have sequenced the genome of Shigella flexneri serotype 2a, the most prevalent species and serotype that causes bacillary dysentery or shigellosis in man. The whole genome is composed of a 4 607 203 bp chromosome and a 221 618 bp virulence plasmid, designated pCP301. While the plasmid shows minor divergence from that sequenced in serotype 5a, striking characteristics of the chromosome have been revealed. The S.flexneri chromosome has, astonishingly, 314 IS elements, more than 7-fold over those possessed by its close relatives, the non-pathogenic K12 strain and enterohemorrhagic O157:H7 strain of Escherichia coli. There are 13 translocations and inversions compared with the E.coli sequences, all involve a segment larger than 5 kb, and most are associated with deletions or acquired DNA sequences, of which several are likely to be bacteriophage-transmitted pathogenicity islands. Furthermore, S.flexneri, resembling another human-restricted enteric pathogen, Salmonella typhi, also has hundreds of pseudogenes compared with the E.coli strains. All of these could be subjected to investigations towards novel preventative and treatment strategies against shigellosis.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
50S ribosomal protein L10P0A7J6Details
UDP-N-acetylglucosamine 1-carboxyvinyltransferaseP0A751Details
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseP62619Details
ADP-ribose pyrophosphataseP83844Details
Transaldolase BP0A872Details
Fatty acid metabolism regulator proteinP0A8V9Details
2-dehydro-3-deoxyphosphooctonate aldolaseP0A716Details
Cell division protein ZipAQ83QN9Details
Outer-membrane lipoprotein LolBP61322Details
S-adenosylmethionine synthaseP0A820Details
Aspartate 1-decarboxylaseP0A793Details
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseQ83LD8Details
Threonine--tRNA ligaseP0A8M5Details
Peptide methionine sulfoxide reductase MsrAP0A745Details
Ribose-5-phosphate isomerase AP0A7Z3Details
N-acetylneuraminate lyaseP0A6L6Details
Aquaporin ZO68874Details
Ribosome-recycling factorP0A808Details
Phosphopantetheine adenylyltransferaseP0A6I8Details
Thiosulfate sulfurtransferase GlpEP0A6V7Details
L-fucose isomeraseP69923Details
GTP cyclohydrolase 1P0A6T8Details
Adenylosuccinate synthetaseQ83P33Details
3-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseP0A6Q5Details
Serine hydroxymethyltransferaseP0A827Details
Elongation factor TuQ83JC4Details
Formyl-CoA:oxalate CoA-transferaseP69903Details
Methylglyoxal synthaseP0A733Details
Glucose-1-phosphate thymidylyltransferase 2P61888Details
Aspartate carbamoyltransferase catalytic chainP0A789Details
Thymidylate kinaseP0A721Details
tRNA (guanine-N(1)-)-methyltransferaseP0A876Details
Nicotinate-nucleotide adenylyltransferaseP0A753Details
Trigger factorP0A852Details
Protein RecAP0A7G9Details
ATP-dependent protease ATPase subunit HslUP0A6H7Details
Lipoprotein MxiMP0A1X2Details
Geranyltranstransferase (Farnesyldiphosphate synthase)A0A0H2UXE9Details