Calreticulin

Details

Name
Calreticulin
Kind
protein
Synonyms
  • Calregulin
  • CRP55
  • CRTC
  • Endoplasmic reticulum resident protein 60
  • ERp60
  • grp60
  • HACBP
Gene Name
CALR
UniProtKB Entry
P27797Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0011528|Calreticulin
MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDE
EKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQT
DMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDN
TYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDWDKPE
HIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYS
PDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDK
QDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL
Number of residues
417
Molecular Weight
48141.2
Theoretical pI
4.04
GO Classification
Functions
complement component C1q complex binding / molecular sequestering activity / nuclear androgen receptor binding / nuclear export signal receptor activity / protein folding chaperone / protein-folding chaperone binding / RNA binding
Processes
cellular response to electrical stimulus / cellular response to virus / ERAD pathway / intracellular calcium ion homeostasis / negative regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / negative regulation of trophoblast cell migration / positive regulation of cell population proliferation / positive regulation of endothelial cell migration / positive regulation of non-canonical NF-kappaB signal transduction / regulation of DNA-templated transcription / response to glycoside / response to xenobiotic stimulus
Components
collagen-containing extracellular matrix / cortical granule / cytolytic granule / endoplasmic reticulum membrane / endoplasmic reticulum quality control compartment / endoplasmic reticulum-Golgi intermediate compartment membrane / lumenal side of endoplasmic reticulum membrane / nuclear envelope / phagocytic vesicle membrane / ribosome
General Function
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity)
Specific Function
Calcium ion binding
Pfam Domain Function
Signal Regions
1-17
Transmembrane Regions
Not Available
Cellular Location
Endoplasmic reticulum lumen
Gene sequence
>lcl|BSEQ0011529|Calreticulin (CALR)
ATGCTGCTATCCGTGCCGCTGCTGCTCGGCCTCCTCGGCCTGGCCGTCGCCGAGCCTGCC
GTCTACTTCAAGGAGCAGTTTCTGGACGGAGACGGGTGGACTTCCCGCTGGATCGAATCC
AAACACAAGTCAGATTTTGGCAAATTCGTTCTCAGTTCCGGCAAGTTCTACGGTGACGAG
GAGAAAGATAAAGGTTTGCAGACAAGCCAGGATGCACGCTTTTATGCTCTGTCGGCCAGT
TTCGAGCCTTTCAGCAACAAAGGCCAGACGCTGGTGGTGCAGTTCACGGTGAAACATGAG
CAGAACATCGACTGTGGGGGCGGCTATGTGAAGCTGTTTCCTAATAGTTTGGACCAGACA
GACATGCACGGAGACTCAGAATACAACATCATGTTTGGTCCCGACATCTGTGGCCCTGGC
ACCAAGAAGGTTCATGTCATCTTCAACTACAAGGGCAAGAACGTGCTGATCAACAAGGAC
ATCCGTTGCAAGGATGATGAGTTTACACACCTGTACACACTGATTGTGCGGCCAGACAAC
ACCTATGAGGTGAAGATTGACAACAGCCAGGTGGAGTCCGGCTCCTTGGAAGACGATTGG
GACTTCCTGCCACCCAAGAAGATAAAGGATCCTGATGCTTCAAAACCGGAAGACTGGGAT
GAGCGGGCCAAGATCGATGATCCCACAGACTCCAAGCCTGAGGACTGGGACAAGCCCGAG
CATATCCCTGACCCTGATGCTAAGAAGCCCGAGGACTGGGATGAAGAGATGGACGGAGAG
TGGGAACCCCCAGTGATTCAGAACCCTGAGTACAAGGGTGAGTGGAAGCCCCGGCAGATC
GACAACCCAGATTACAAGGGCACTTGGATCCACCCAGAAATTGACAACCCCGAGTATTCT
CCCGATCCCAGTATCTATGCCTATGATAACTTTGGCGTGCTGGGCCTGGACCTCTGGCAG
GTCAAGTCTGGCACCATCTTTGACAACTTCCTCATCACCAACGATGAGGCATACGCTGAG
GAGTTTGGCAACGAGACGTGGGGCGTAACAAAGGCAGCAGAGAAACAAATGAAGGACAAA
CAGGACGAGGAGCAGAGGCTTAAGGAGGAGGAAGAAGACAAGAAACGCAAAGAGGAGGAG
GAGGCAGAGGACAAGGAGGATGATGAGGACAAAGATGAGGATGAGGAGGATGAGGAGGAC
AAGGAGGAAGATGAGGAGGAAGATGTCCCCGGCCAGGCCAAGGACGAGCTGTAG
Chromosome Location
19
Locus
19p13.13
External Identifiers
ResourceLink
UniProtKB IDP27797
UniProtKB Entry NameCALR_HUMAN
GenBank Protein ID337487
GenBank Gene IDM32294
GeneCard IDCALR
GenAtlas IDCALR
HGNC IDHGNC:1455
PDB ID(s)2CLR, 3DOW, 3POS, 3POW, 5LK5, 5V90, 6ENY, 7QPD, 8TZO, 8TZR
KEGG IDhsa:811
NCBI Gene ID811
General References
  1. McCauliffe DP, Lux FA, Lieu TS, Sanz I, Hanke J, Newkirk MM, Bachinski LL, Itoh Y, Siciliano MJ, Reichlin M, et al.: Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen. J Clin Invest. 1990 May;85(5):1379-91. [Article]
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  3. McCauliffe DP, Yang YS, Wilson J, Sontheimer RD, Capra JD: The 5'-flanking region of the human calreticulin gene shares homology with the human GRP78, GRP94, and protein disulfide isomerase promoters. J Biol Chem. 1992 Feb 5;267(4):2557-62. [Article]
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  9. Rojiani MV, Finlay BB, Gray V, Dedhar S: In vitro interaction of a polypeptide homologous to human Ro/SS-A antigen (calreticulin) with a highly conserved amino acid sequence in the cytoplasmic domain of integrin alpha subunits. Biochemistry. 1991 Oct 15;30(41):9859-66. [Article]
  10. Krause KH, Simmerman HK, Jones LR, Campbell KP: Sequence similarity of calreticulin with a Ca2(+)-binding protein that co-purifies with an Ins(1,4,5)P3-sensitive Ca2+ store in HL-60 cells. Biochem J. 1990 Sep 1;270(2):545-8. [Article]
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Associated Data

Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Antihemophilic factor, human recombinantapproved, investigationalunknowntargetchaperoneDetails
TenecteplaseapprovedunknowntargetDetails
LanoteplaseinvestigationalunknowntargetDetails
Melatoninapproved, nutraceutical, vet_approvedunknowntargetDetails
Calcium citrateapproved, investigationalnotargetligandDetails
Calcium PhosphateapprovednotargetligandDetails
Copperapproved, investigationalunknowntargetDetails
Lonoctocog alfaapproved, investigationalunknowntargetchaperoneDetails
Moroctocog alfaapprovedunknowntargetchaperoneDetails
Ferric cationapprovedunknowncarrierbinderDetails
Calcium phosphate dihydrateapprovednotargetDetails
Tetraferric tricitrate decahydrateapprovedunknowncarrierbinderDetails