Uncovering global SUMOylation signaling networks in a site-specific manner.

Article Details

Citation

Hendriks IA, D'Souza RC, Yang B, Verlaan-de Vries M, Mann M, Vertegaal AC

Uncovering global SUMOylation signaling networks in a site-specific manner.

Nat Struct Mol Biol. 2014 Oct;21(10):927-36. doi: 10.1038/nsmb.2890. Epub 2014 Sep 14.

PubMed ID
25218447 [ View in PubMed
]
Abstract

SUMOylation is a reversible post-translational modification essential for genome stability. Using high-resolution MS, we have studied global SUMOylation in human cells in a site-specific manner, identifying a total of >4,300 SUMOylation sites in >1,600 proteins. To our knowledge, this is the first time that >1,000 SUMOylation sites have been identified under standard growth conditions. We quantitatively studied SUMOylation dynamics in response to SUMO protease inhibition, proteasome inhibition and heat shock. Many SUMOylated lysines have previously been reported to be ubiquitinated, acetylated or methylated, thus indicating cross-talk between SUMO and other post-translational modifications. We identified 70 phosphorylation and four acetylation events in proximity to SUMOylation sites, and we provide evidence for acetylation-dependent SUMOylation of endogenous histone H3. SUMOylation regulates target proteins involved in all nuclear processes including transcription, DNA repair, chromatin remodeling, precursor-mRNA splicing and ribosome assembly.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
B-cell receptor CD22P20273Details
DNA topoisomerase 2-alphaP11388Details
CalpastatinP20810Details
DNA topoisomerase 1P11387Details
78 kDa glucose-regulated proteinP11021Details
Proto-oncogene c-FosP01100Details
Poly [ADP-ribose] polymerase 1P09874Details
Keratin, type II cytoskeletal 8P05787Details
Histone deacetylase 1Q13547Details
Histone deacetylase 2Q92769Details
DNA topoisomerase 2-betaQ02880Details
Keratin, type II cytoskeletal 7P08729Details
Nuclear receptor coactivator 2Q15596Details
Nuclear receptor-interacting protein 1P48552Details
Heat shock cognate 71 kDa proteinP11142Details
60S ribosomal protein L26-like 1Q9UNX3Details
Calcium/calmodulin-dependent protein kinase type 1DQ8IU85Details
Jun dimerization protein 2Q8WYK2Details
Cyclic AMP-dependent transcription factor ATF-4P18848Details
CCAAT/enhancer-binding protein betaP17676Details
Protein PMLP29590Details
Nucleolar and coiled-body phosphoprotein 1Q14978Details
Heterogeneous nuclear ribonucleoproteins A2/B1P22626Details
Heterogeneous nuclear ribonucleoprotein LP14866Details
Splicing factor, proline- and glutamine-richP23246Details
Stress-induced-phosphoprotein 1P31948Details
Hepatoma-derived growth factorP51858Details
Ran-specific GTPase-activating proteinP43487Details
Heterogeneous nuclear ribonucleoprotein KP61978Details
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1Q969G3Details
Chromobox protein homolog 5P45973Details
Filamin-AP21333Details
NucleophosminP06748Details
60S ribosomal protein L14P50914Details
Probable ATP-dependent RNA helicase DDX5P17844Details
Splicing factor 3B subunit 1O75533Details
Serine/threonine-protein kinase PRP4 homologQ13523Details