Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.

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Wollscheid B, Bausch-Fluck D, Henderson C, O'Brien R, Bibel M, Schiess R, Aebersold R, Watts JD

Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.

Nat Biotechnol. 2009 Apr;27(4):378-86. doi: 10.1038/nbt.1532. Epub 2009 Apr 6.

PubMed ID
19349973 [ View in PubMed
]
Abstract

Although the classification of cell types often relies on the identification of cell surface proteins as differentiation markers, flow cytometry requires suitable antibodies and currently permits detection of only up to a dozen differentiation markers in a single measurement. We use multiplexed mass-spectrometric identification of several hundred N-linked glycosylation sites specifically from cell surface-exposed glycoproteins to phenotype cells without antibodies in an unbiased fashion and without a priori knowledge. We apply our cell surface-capturing (CSC) technology, which covalently labels extracellular glycan moieties on live cells, to the detection and relative quantitative comparison of the cell surface N-glycoproteomes of T and B cells, as well as to monitor changes in the abundance of cell surface N-glycoprotein markers during T-cell activation and the controlled differentiation of embryonic stem cells into the neural lineage. A snapshot view of the cell surface N-glycoproteins will enable detection of panels of N-glycoproteins as potential differentiation markers that are currently not accessible by other means.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Insulin receptorP06213Details
Solute carrier family 22 member 5O76082Details
High affinity cationic amino acid transporter 1P30825Details
Neutral amino acid transporter B(0)Q15758Details
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1P22413Details
Neutral amino acid transporter AP43007Details
Integrin alpha-LP20701Details
Integrin alpha-4P13612Details
Interferon alpha/beta receptor 1P17181Details
Gamma-glutamyltranspeptidase 1P19440Details
Solute carrier family 2, facilitated glucose transporter member 4P14672Details
Integrin beta-2P05107Details
Solute carrier family 2, facilitated glucose transporter member 1P11166Details
Intercellular adhesion molecule 1P05362Details
5'-nucleotidaseP21589Details
Major prion proteinP04156Details
Equilibrative nucleoside transporter 1Q99808Details
Choline transporter-like protein 2Q8IWA5Details
Voltage-dependent calcium channel subunit alpha-2/delta-2Q9NY47Details
Disintegrin and metalloproteinase domain-containing protein 10O14672Details
Anthrax toxin receptor 1Q9H6X2Details
Receptor-type tyrosine-protein phosphatase SQ13332Details
Integrin beta-1P05556Details
Integrin alpha-VP06756Details
T-cell receptor alpha chain C regionP01848Details
Endothelin-converting enzyme 1P42892Details
Antigen-presenting glycoprotein CD1dP15813Details
Sodium bicarbonate cotransporter 3Q9Y6M7Details
Transferrin receptor protein 1P02786Details
Sodium/potassium-transporting ATPase subunit beta-1P05026Details
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1P28907Details
Integrin alpha-5P08648Details
Sodium- and chloride-dependent taurine transporterP31641Details
P2Y purinoceptor 8Q86VZ1Details
Zinc transporter ZIP6Q13433Details
Zinc transporter ZIP14Q15043Details
Leucyl-cystinyl aminopeptidaseQ9UIQ6Details
HLA class I histocompatibility antigen, alpha chain EP13747Details