Escherichia coli proteome analysis using the gene-protein database.
Article Details
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VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC
Escherichia coli proteome analysis using the gene-protein database.
Electrophoresis. 1997 Aug;18(8):1243-51.
- PubMed ID
- 9298644 [ View in PubMed]
- Abstract
The gene-protein database of Escherichia coli is a collection of data, largely generated from the separation of complex mixtures of cellular proteins on two-dimensional (2-D) polyacrylamide gel electrophoresis. The database currently contains about 1600 protein spots. The data are comprised of both identification information for many of these proteins and data on how the level or synthesis rates of proteins vary under different growth conditions. Three projects are underway to further elucidate the E. coli proteome including a project to localize on 2-D gels all of the open reading framed encoded by the E. coli chromosome, a project to determine the condition(s) under which each open reading frame is expressed and a project to determine the abundance and location of each protein in the cell. Applications for proteome databases for cell modeling are discussed and examples of applications in therapeutic drug discovery are given.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID Malate dehydrogenase P61889 Details D-lactate dehydrogenase P06149 Details 30S ribosomal protein S9 P0A7X3 Details 30S ribosomal protein S10 P0A7R5 Details DNA-directed RNA polymerase subunit beta P0A8V2 Details Glutathione reductase P06715 Details DNA-directed RNA polymerase subunit alpha P0A7Z4 Details DNA polymerase I P00582 Details Fumarate reductase flavoprotein subunit P00363 Details Pyruvate dehydrogenase [ubiquinone] P07003 Details DNA gyrase subunit B P0AES6 Details 3-oxoacyl-[acyl-carrier-protein] synthase 1 P0A953 Details Aspartate aminotransferase P00509 Details Adenylate kinase P69441 Details Isocitrate dehydrogenase [NADP] P08200 Details Beta-lactamase P00811 Details Deoxyuridine 5'-triphosphate nucleotidohydrolase P06968 Details Beta-galactosidase P00722 Details Thymidylate synthase P0A884 Details Methionine--tRNA ligase P00959 Details UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase P22188 Details Tryptophan biosynthesis protein TrpCF P00909 Details Outer membrane protein F P02931 Details Glycerol kinase P0A6F3 Details Methionine synthase P13009 Details DNA-3-methyladenine glycosylase 1 P05100 Details L-arabinose-binding periplasmic protein P02924 Details Biotin synthase P12996 Details Formate acetyltransferase 1 P09373 Details Histidine--tRNA ligase P60906 Details Phosphoenolpyruvate carboxylase P00864 Details Diaminopimelate decarboxylase P00861 Details Ornithine carbamoyltransferase chain I P04391 Details Rhamnulose-1-phosphate aldolase P32169 Details Alkaline phosphatase P00634 Details Fumarate hydratase class II P05042 Details Ribose import binding protein RbsB P02925 Details DNA topoisomerase 1 P06612 Details Maltose-binding periplasmic protein P0AEX9 Details Pyruvate dehydrogenase E1 component P0AFG8 Details 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase P0AF12 Details Dihydrofolate reductase P0ABQ4 Details Methionine aminopeptidase P0AE18 Details cAMP-activated global transcriptional regulator CRP P0ACJ8 Details Branched-chain-amino-acid aminotransferase P0AB80 Details Soluble lytic murein transglycosylase P0AGC3 Details Chemotaxis protein CheY P0AE67 Details AMP nucleosidase P0AE12 Details Tyrosine--tRNA ligase P0AGJ9 Details Arabinose operon regulatory protein P0A9E0 Details Single-stranded DNA-binding protein P0AGE0 Details Amidophosphoribosyltransferase P0AG16 Details Outer membrane protein A P0A910 Details 2-amino-3-ketobutyrate coenzyme A ligase P0AB77 Details Glyceraldehyde-3-phosphate dehydrogenase A P0A9B2 Details D-galactose-binding periplasmic protein P0AEE5 Details D-alanyl-D-alanine carboxypeptidase DacA P0AEB2 Details Thioredoxin reductase P0A9P4 Details KHG/KDPG aldolase P0A955 Details DNA polymerase III subunit epsilon P03007 Details Lactose operon repressor P03023 Details DNA polymerase III subunit tau P06710 Details Ecotin P23827 Details Flavodoxin-1 P61949 Details Glutamate decarboxylase alpha P69908 Details Cystathionine gamma-synthase P00935 Details Aspartate carbamoyltransferase catalytic subunit P0A786 Details Succinate dehydrogenase flavoprotein subunit P0AC41 Details 50S ribosomal protein L4 P60723 Details DNA-directed RNA polymerase subunit beta' P0A8T7 Details DNA polymerase III subunit beta P0A988 Details Fumarate reductase iron-sulfur subunit P0AC47 Details ATP-dependent Clp protease proteolytic subunit P0A6G7 Details 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase P0A6Q3 Details Methionyl-tRNA formyltransferase P23882 Details Colicin I receptor P17315 Details Thiol:disulfide interchange protein DsbA P0AEG4 Details 30S ribosomal protein S8 P0A7W7 Details DNA gyrase subunit A P0AES4 Details Outer membrane porin C P06996 Details UvrABC system protein A P0A698 Details Cold shock protein CspA P0A9X9 Details Protein RecA P0A7G6 Details