Escherichia coli proteome analysis using the gene-protein database.

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VanBogelen RA, Abshire KZ, Moldover B, Olson ER, Neidhardt FC

Escherichia coli proteome analysis using the gene-protein database.

Electrophoresis. 1997 Aug;18(8):1243-51.

PubMed ID
9298644 [ View in PubMed
]
Abstract

The gene-protein database of Escherichia coli is a collection of data, largely generated from the separation of complex mixtures of cellular proteins on two-dimensional (2-D) polyacrylamide gel electrophoresis. The database currently contains about 1600 protein spots. The data are comprised of both identification information for many of these proteins and data on how the level or synthesis rates of proteins vary under different growth conditions. Three projects are underway to further elucidate the E. coli proteome including a project to localize on 2-D gels all of the open reading framed encoded by the E. coli chromosome, a project to determine the condition(s) under which each open reading frame is expressed and a project to determine the abundance and location of each protein in the cell. Applications for proteome databases for cell modeling are discussed and examples of applications in therapeutic drug discovery are given.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Malate dehydrogenaseP61889Details
D-lactate dehydrogenaseP06149Details
30S ribosomal protein S9P0A7X3Details
30S ribosomal protein S10P0A7R5Details
DNA-directed RNA polymerase subunit betaP0A8V2Details
Glutathione reductaseP06715Details
DNA-directed RNA polymerase subunit alphaP0A7Z4Details
DNA polymerase IP00582Details
Fumarate reductase flavoprotein subunitP00363Details
Pyruvate dehydrogenase [ubiquinone]P07003Details
DNA gyrase subunit BP0AES6Details
3-oxoacyl-[acyl-carrier-protein] synthase 1P0A953Details
Aspartate aminotransferaseP00509Details
Adenylate kinaseP69441Details
Isocitrate dehydrogenase [NADP]P08200Details
Beta-lactamaseP00811Details
Deoxyuridine 5'-triphosphate nucleotidohydrolaseP06968Details
Beta-galactosidaseP00722Details
Thymidylate synthaseP0A884Details
Methionine--tRNA ligaseP00959Details
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseP22188Details
Tryptophan biosynthesis protein TrpCFP00909Details
Outer membrane protein FP02931Details
Glycerol kinaseP0A6F3Details
Methionine synthaseP13009Details
DNA-3-methyladenine glycosylase 1P05100Details
L-arabinose-binding periplasmic proteinP02924Details
Biotin synthaseP12996Details
Formate acetyltransferase 1P09373Details
Histidine--tRNA ligaseP60906Details
Phosphoenolpyruvate carboxylaseP00864Details
Diaminopimelate decarboxylaseP00861Details
Ornithine carbamoyltransferase chain IP04391Details
Rhamnulose-1-phosphate aldolaseP32169Details
Alkaline phosphataseP00634Details
Fumarate hydratase class IIP05042Details
Ribose import binding protein RbsBP02925Details
DNA topoisomerase 1P06612Details
Maltose-binding periplasmic proteinP0AEX9Details
Pyruvate dehydrogenase E1 componentP0AFG8Details
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseP0AF12Details
Dihydrofolate reductaseP0ABQ4Details
Methionine aminopeptidaseP0AE18Details
cAMP-activated global transcriptional regulator CRPP0ACJ8Details
Branched-chain-amino-acid aminotransferaseP0AB80Details
Soluble lytic murein transglycosylaseP0AGC3Details
Chemotaxis protein CheYP0AE67Details
AMP nucleosidaseP0AE12Details
Tyrosine--tRNA ligaseP0AGJ9Details
Arabinose operon regulatory proteinP0A9E0Details
Single-stranded DNA-binding proteinP0AGE0Details
AmidophosphoribosyltransferaseP0AG16Details
Outer membrane protein AP0A910Details
2-amino-3-ketobutyrate coenzyme A ligaseP0AB77Details
Glyceraldehyde-3-phosphate dehydrogenase AP0A9B2Details
D-galactose-binding periplasmic proteinP0AEE5Details
D-alanyl-D-alanine carboxypeptidase DacAP0AEB2Details
Thioredoxin reductaseP0A9P4Details
KHG/KDPG aldolaseP0A955Details
DNA polymerase III subunit epsilonP03007Details
Lactose operon repressorP03023Details
DNA polymerase III subunit tauP06710Details
EcotinP23827Details
Flavodoxin-1P61949Details
Glutamate decarboxylase alphaP69908Details
Cystathionine gamma-synthaseP00935Details
Aspartate carbamoyltransferase catalytic subunitP0A786Details
Succinate dehydrogenase flavoprotein subunitP0AC41Details
50S ribosomal protein L4P60723Details
DNA-directed RNA polymerase subunit beta'P0A8T7Details
DNA polymerase III subunit betaP0A988Details
Fumarate reductase iron-sulfur subunitP0AC47Details
ATP-dependent Clp protease proteolytic subunitP0A6G7Details
3-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseP0A6Q3Details
Methionyl-tRNA formyltransferaseP23882Details
Colicin I receptorP17315Details
Thiol:disulfide interchange protein DsbAP0AEG4Details
30S ribosomal protein S8P0A7W7Details
DNA gyrase subunit AP0AES4Details
Outer membrane porin CP06996Details
UvrABC system protein AP0A698Details
Cold shock protein CspAP0A9X9Details
Protein RecAP0A7G6Details