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Neostigmine
Identification
- Name
- Neostigmine
- Accession Number
- DB01400
- Description
A cholinesterase inhibitor used in the treatment of myasthenia gravis and to reverse the effects of muscle relaxants such as gallamine and tubocurarine. Neostigmine, unlike physostigmine, does not cross the blood-brain barrier.
- Type
- Small Molecule
- Groups
- Approved, Vet approved
- Structure
Structure for Neostigmine (DB01400)
- Weight
- Average: 223.2915
Monoisotopic: 223.144652862 - Chemical Formula
- C12H19N2O2
- Synonyms
- (m-Hydroxyphenyl)trimethylammonium dimethylcarbamate
- 3-Trimethylammoniumphenyl N,N-dimethylcarbamate
- Eustigmin
- Eustigmine
- m-Trimethylammoniumphenyldimethylcarbamate
- Neostigmina
Pharmacology
- Indication
Neostigmine is used for the symptomatic treatment of myasthenia gravis by improving muscle tone.
- Associated Conditions
- Contraindications & Blackbox Warnings
Learn about our commercial Contraindications & Blackbox Warnings data.
Learn More- Pharmacodynamics
Neostigmine is a cholinesterase inhibitor used in the treatment of myasthenia gravis and to reverse the effects of muscle relaxants such as gallamine and tubocurarine. Neostigmine, unlike physostigmine, does not cross the blood-brain barrier. By inhibiting acetylcholinesterase, more acetylcholine is available in the synapse, therefore, more of it can bind to the fewer receptors present in myasthenia gravis and can better trigger muscular contraction.
- Mechanism of action
Neostigmine is a parasympathomimetic, specifically, a reversible cholinesterase inhibitor. The drug inhibits acetylcholinesterase which is responsible for the degredation of acetylcholine. So, with acetylcholinesterase inhibited, more acetylcholine is present By interfering with the breakdown of acetylcholine, neostigmine indirectly stimulates both nicotinic and muscarinic receptors which are involved in muscle contraction.. It does not cross the blood-brain barrier.
Target Actions Organism AAcetylcholinesterase inhibitorHumans - Absorption
Neostigmine bromide is poorly absorbed from the gastrointestinal tract following oral administration
- Volume of distribution
- Not Available
- Protein binding
Protein binding to human serum albumin ranges from 15 to 25 percent.
- Metabolism
Neostigmine undergoes hydrolysis by cholinesterase and is also metabolized by microsomal enzymes in the liver.
- Route of elimination
- Not Available
- Half-life
The half-life ranged from 42 to 60 minutes with a mean half-life of 52 minutes.
- Clearance
- Not Available
- Adverse Effects
Learn about our commercial Adverse Effects data.
Learn More- Toxicity
Overdosage of Neostigmine can cause cholinergic crisis, which is characterized by increasing muscle weakness, and through involvement of the muscles of respiration, may result in death. The LD 50 of neostigmine methylsulfate in mice is 0.3 ± 0.02 mg/kg intravenously, 0.54 ± 0.03 mg/kg subcutaneously, and 0.395 ± 0.025 mg/kg intramuscularly; in rats the LD 50 is 0.315 ± 0.019 mg/kg intravenously, 0.445 ± 0.032 mg/kg subcutaneously, and 0.423 ± 0.032 mg/kg intramuscularly.
- Affected organisms
- Humans and other mammals
- Pathways
- Not Available
- Pharmacogenomic Effects/ADRs
- Not Available
Interactions
- Drug Interactions
- This information should not be interpreted without the help of a healthcare provider. If you believe you are experiencing an interaction, contact a healthcare provider immediately. The absence of an interaction does not necessarily mean no interactions exist.
Drug Interaction Unlock Additional DataAcebutolol Neostigmine may increase the bradycardic activities of Acebutolol. Acetylcholine The risk or severity of adverse effects can be increased when Neostigmine is combined with Acetylcholine. Aclidinium Neostigmine may increase the neuromuscular blocking activities of Aclidinium. Amantadine The therapeutic efficacy of Amantadine can be decreased when used in combination with Neostigmine. Amifampridine The risk or severity of adverse effects can be increased when Neostigmine is combined with Amifampridine. Amitriptyline The therapeutic efficacy of Amitriptyline can be decreased when used in combination with Neostigmine. Amobarbital The therapeutic efficacy of Amobarbital can be decreased when used in combination with Neostigmine. Amoxapine The therapeutic efficacy of Amoxapine can be decreased when used in combination with Neostigmine. Anisotropine methylbromide The therapeutic efficacy of Anisotropine methylbromide can be decreased when used in combination with Neostigmine. Aripiprazole The therapeutic efficacy of Aripiprazole can be decreased when used in combination with Neostigmine. Additional Data Available- Extended DescriptionExtended Description
Extended description of the mechanism of action and particular properties of each drug interaction.
Learn more - Severity
- Evidence Level
- ActionAction
An effect category for each drug interaction. Know how this interaction affects the subject drug.
Learn more
- Food Interactions
- No interactions found.
Products
- Product Ingredients
Ingredient UNII CAS InChI Key Neostigmine bromide 005SYP50G5 114-80-7 LULNWZDBKTWDGK-UHFFFAOYSA-M Neostigmine methylsulfate 98IMH7M386 51-60-5 OSZNNLWOYWAHSS-UHFFFAOYSA-M - International/Other Brands
- Vagostigmin
- Brand Name Prescription Products
Name Dosage Strength Route Labeller Marketing Start Marketing End Region Image Unlock Additional DataBloxiverz Injection 1 mg/1mL Intravenous BluePoint Laboratories 2017-06-30 Not applicable US 
Bloxiverz Injection 0.5 mg/1mL Intravenous Avadel Legacy Pharmaceuticals, Llc 2013-07-24 2018-12-31 US Bloxiverz Injection 1 mg/1mL Intravenous Avadel Legacy Pharmaceuticals, Llc 2013-07-24 Not applicable US Bloxiverz Injection 0.5 mg/1mL Intravenous Avadel Legacy Pharmaceuticals, Llc 2013-07-24 Not applicable US Bloxiverz Injection 1 mg/1mL Intravenous Avadel Legacy Pharmaceuticals, Llc 2013-07-24 Not applicable US Neostigmine Methylsuflate Injection, solution 1 mg/1mL Intramuscular; Intravenous; Subcutaneous General Injectables & Vaccines 2010-08-01 2014-08-31 US Neostigmine Methylsulfate Injection, solution 1 mg/1mL Intravenous Cantrell Drug Company 2015-02-03 2017-12-06 US Neostigmine Methylsulfate Injection, solution 1 mg/1mL Intravenous REMEDYREPACK INC. 2015-10-19 2016-10-19 US Neostigmine Methylsulfate Injection, solution 1 mg/1mL Intramuscular; Intravenous; Subcutaneous Cardinal Health 2003-01-16 2014-08-31 US Neostigmine Methylsulfate Injection, solution 1 mg/1mL Intramuscular; Intravenous; Subcutaneous Fresenius Kabi 2000-09-01 2016-11-18 US Additional Data Available- Application NumberApplication Number
A unique ID assigned by the FDA when a product is submitted for approval by the labeller.
Learn more - Product CodeProduct Code
A governmentally-recognized ID which uniquely identifies the product within its regulatory market.
Learn more
- Generic Prescription Products
Name Dosage Strength Route Labeller Marketing Start Marketing End Region Image Unlock Additional DataNeostigmine Injection 0.5 mg/1mL Intravenous Dr.Reddy's Laboratories Inc 2018-09-04 Not applicable US Neostigmine Injection 1 mg/1mL Intravenous Medical Purchasing Solutions, Llc 2018-09-04 Not applicable US Neostigmine Injection 1 mg/1mL Intravenous Dr.Reddy's Laboratories Inc 2018-09-04 Not applicable US Neostigmine Methylsulfate Injection 1 mg/1mL Intravenous Renaissance Ssa, Llc 2019-12-31 2019-12-31 US Neostigmine methylsulfate Injection 1 mg/1mL Intravenous Xiromed Llc 2020-09-09 Not applicable US Neostigmine methylsulfate Injection 0.5 mg/1mL Intravenous Gland Pharma Limited 2019-10-21 Not applicable US Neostigmine methylsulfate Injection 0.5 mg/1mL Intravenous Amneal Pharmaceuticals LLC 2018-06-20 Not applicable US Neostigmine Methylsulfate Injection 1 mg/1mL Intravenous AMERICAN REGENT, INC. 2018-05-14 Not applicable US Neostigmine Methylsulfate Injection 0.5 mg/1mL Intravenous Amphastar Pharmaceuticals, Inc. 2017-09-25 Not applicable US Neostigmine Methylsulfate Injection 0.5 mg/1mL Intravenous Par Pharmaceutical, Inc. 2017-04-26 Not applicable US Additional Data Available- Application NumberApplication Number
A unique ID assigned by the FDA when a product is submitted for approval by the labeller.
Learn more - Product CodeProduct Code
A governmentally-recognized ID which uniquely identifies the product within its regulatory market.
Learn more
- Unapproved/Other Products
Name Ingredients Dosage Route Labeller Marketing Start Marketing End Region Image Neostigmine Methylsuflate Neostigmine methylsulfate (1 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous General Injectables & Vaccines 2010-08-01 2014-08-31 US Neostigmine Methylsulfate Neostigmine methylsulfate (1 mg/1mL) Injection, solution Intravenous Cantrell Drug Company 2015-02-03 2017-12-06 US Neostigmine Methylsulfate Neostigmine methylsulfate (1 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous Cardinal Health 2003-01-16 2014-08-31 US Neostigmine Methylsulfate Neostigmine methylsulfate (1 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous Fresenius Kabi 2000-09-01 2016-11-18 US Neostigmine Methylsulfate Neostigmine methylsulfate (1 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous Claris Lifesciences Limited 2009-10-09 2010-11-04 US Neostigmine Methylsulfate Neostigmine methylsulfate (1 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous General Injectables & Vaccines 2011-08-19 2012-11-30 US Neostigmine Methylsulfate Neostigmine methylsulfate (0.5 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous Claris Lifesciences, Inc. 2009-10-07 2010-11-04 US Neostigmine Methylsulfate Neostigmine methylsulfate (1 mg/1mL) Injection Intramuscular; Intravenous; Subcutaneous West-Ward Pharmaceuticals Corp. 1973-01-01 2015-08-31 US Neostigmine Methylsulfate Neostigmine methylsulfate (0.5 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous American Regent 2003-01-17 2016-10-17 US Neostigmine Methylsulfate Neostigmine methylsulfate (5 mg/1mL) Injection, solution Intramuscular; Intravenous; Subcutaneous Fresenius Kabi 2000-10-18 2016-11-18 US
Categories
- ATC Codes
- N07AA01 — Neostigmine
- N07AA — Anticholinesterases
- N07A — PARASYMPATHOMIMETICS
- N07 — OTHER NERVOUS SYSTEM DRUGS
- N — NERVOUS SYSTEM
- S01EB — Parasympathomimetics
- S01E — ANTIGLAUCOMA PREPARATIONS AND MIOTICS
- S01 — OPHTHALMOLOGICALS
- S — SENSORY ORGANS
- Drug Categories
- Amines
- Ammonium Compounds
- Antiglaucoma Preparations and Miotics
- Autonomic Agents
- Cholinergic Agents
- Cholinesterase Inhibitors
- Enzyme Inhibitors
- Nervous System
- Neurotransmitter Agents
- Nitrogen Compounds
- Onium Compounds
- Ophthalmologicals
- Parasympathomemetic (Cholinergic) Agents
- Parasympathomimetics
- Peripheral Nervous System Agents
- Phenylammonium Compounds
- Quaternary Ammonium Compounds
- Sensory Organs
- Chemical TaxonomyProvided by Classyfire
- Description
- This compound belongs to the class of organic compounds known as phenoxy compounds. These are aromatic compounds contaning a phenoxy group.
- Kingdom
- Organic compounds
- Super Class
- Benzenoids
- Class
- Benzene and substituted derivatives
- Sub Class
- Phenoxy compounds
- Direct Parent
- Phenoxy compounds
- Alternative Parents
- Aniline and substituted anilines / Quaternary ammonium salts / Carbamate esters / Organic carbonic acids and derivatives / Organopnictogen compounds / Organic salts / Organic oxides / Hydrocarbon derivatives / Carbonyl compounds / Amines show 1 more
- Substituents
- Amine / Aniline or substituted anilines / Aromatic homomonocyclic compound / Carbamic acid ester / Carbonic acid derivative / Carbonyl group / Hydrocarbon derivative / Organic cation / Organic nitrogen compound / Organic oxide show 7 more
- Molecular Framework
- Aromatic homomonocyclic compounds
- External Descriptors
- quaternary ammonium ion (CHEBI:7514)
Chemical Identifiers
- UNII
- 3982TWQ96G
- CAS number
- 59-99-4
- InChI Key
- ALWKGYPQUAPLQC-UHFFFAOYSA-N
- InChI
- InChI=1S/C12H19N2O2/c1-13(2)12(15)16-11-8-6-7-10(9-11)14(3,4)5/h6-9H,1-5H3/q+1
- IUPAC Name
- 3-[(dimethylcarbamoyl)oxy]-N,N,N-trimethylanilinium
- SMILES
- CN(C)C(=O)OC1=CC(=CC=C1)[N+](C)(C)C
References
- General References
- Not Available
- External Links
- Human Metabolome Database
- HMDB0015472
- KEGG Drug
- D08261
- KEGG Compound
- C07258
- PubChem Compound
- 4456
- PubChem Substance
- 46509161
- ChemSpider
- 4301
- BindingDB
- 50022775
- 7315
- ChEBI
- 7514
- ChEMBL
- CHEMBL278020
- ZINC
- ZINC000000001792
- Therapeutic Targets Database
- DAP000563
- PharmGKB
- PA450611
- RxList
- RxList Drug Page
- Drugs.com
- Drugs.com Drug Page
- Wikipedia
- Neostigmine
- AHFS Codes
- 12:04.00 — Parasympathomemetic (Cholinergic) Agents
- MSDS
- Download (74.7 KB)
Clinical Trials
- Clinical Trials
Phase Status Purpose Conditions Count 4 Active Not Recruiting Prevention Residual Paralysis, Post Anesthesia 1 4 Active Not Recruiting Supportive Care Cancer, Bladder / Malignant Neoplasms of Urinary Tract 1 4 Active Not Recruiting Supportive Care Muscle Relaxation 1 4 Active Not Recruiting Treatment Anaesthesia therapy / Neuromuscular Blockade 1 4 Active Not Recruiting Treatment Malignant Neoplasms of Digestive Organs / Malignant Neoplasms of Female Genital Organs / Malignant Neoplasms of Male Genital Organs / Malignant Neoplasms of Urinary Tract 1 4 Completed Basic Science Electromyography / Respiratory Muscles 1 4 Completed Diagnostic Intraocular Pressure Changes During Tracheal Extubation 1 4 Completed Diagnostic Residual Neuromuscular Block 1 4 Completed Diagnostic Sphincter of Oddi Dysfunction 1 4 Completed Diagnostic Spinal Cord Injuries (SCI) 1
Pharmacoeconomics
- Manufacturers
- Not Available
- Packagers
- American Regent
- Pharmedium
- Spectrum Pharmaceuticals
- Valeant Ltd.
- Dosage Forms
Form Route Strength Injection Intravenous 0.5 mg/1mL Injection Intravenous 1 mg/1mL Injection, solution Parenteral 0.5 MG/ML Injection, solution Intramuscular; Intravenous; Subcutaneous 0.5 mg/ml Injection 0.5 mg/ml Solution Intramuscular; Intravenous 0.5 mg Solution Intramuscular; Intravenous; Subcutaneous 0.5 mg Injection Intramuscular; Intravenous; Subcutaneous 0.5 mg/1mL Injection Intramuscular; Intravenous; Subcutaneous 1 mg/1mL Injection, solution Intramuscular; Intravenous; Subcutaneous 0.5 mg/1mL Injection, solution Intramuscular; Intravenous; Subcutaneous 1 mg/1mL Injection, solution Intramuscular; Intravenous; Subcutaneous 5 mg/1mL Injection, solution Intravenous 0.5 mg/1mL Injection, solution Intravenous 1 mg/1mL Solution Intramuscular; Intravenous; Subcutaneous Liquid Intramuscular; Intravenous; Subcutaneous Tablet Oral 15 mg/1 Tablet Oral Injection 0.5 mg/1ml - Prices
Unit description Cost Unit Neostigmine bromide powder 96.57USD g Neostigmine ms 5 mg/5 ml syr 1.99USD ml Prostigmin 1:4000 ampul 1.42USD ml Prostigmin 15 mg tablet 1.04USD tablet Neostigmine 1:1000 vial 0.48USD ml Neostigmine 1:2000 vial 0.48USD ml DrugBank does not sell nor buy drugs. Pricing information is supplied for informational purposes only.- Patents
- Not Available
Properties
- State
- Solid
- Experimental Properties
- Not Available
- Predicted Properties
Property Value Source Water Solubility 0.0677 mg/mL ALOGPS logP -1.6 ALOGPS logP -2.2 ChemAxon logS -3.6 ALOGPS Physiological Charge 1 ChemAxon Hydrogen Acceptor Count 1 ChemAxon Hydrogen Donor Count 0 ChemAxon Polar Surface Area 29.54 Å2 ChemAxon Rotatable Bond Count 3 ChemAxon Refractivity 75.28 m3·mol-1 ChemAxon Polarizability 25.08 Å3 ChemAxon Number of Rings 1 ChemAxon Bioavailability 1 ChemAxon Rule of Five Yes ChemAxon Ghose Filter No ChemAxon Veber's Rule Yes ChemAxon MDDR-like Rule No ChemAxon - Predicted ADMET Features
Property Value Probability Human Intestinal Absorption - 0.8576 Blood Brain Barrier + 0.9583 Caco-2 permeable + 0.608 P-glycoprotein substrate Non-substrate 0.8263 P-glycoprotein inhibitor I Non-inhibitor 0.9598 P-glycoprotein inhibitor II Non-inhibitor 0.9279 Renal organic cation transporter Non-inhibitor 0.9181 CYP450 2C9 substrate Non-substrate 0.7197 CYP450 2D6 substrate Non-substrate 0.6869 CYP450 3A4 substrate Substrate 0.622 CYP450 1A2 substrate Non-inhibitor 0.9045 CYP450 2C9 inhibitor Non-inhibitor 0.907 CYP450 2D6 inhibitor Non-inhibitor 0.9367 CYP450 2C19 inhibitor Non-inhibitor 0.9025 CYP450 3A4 inhibitor Non-inhibitor 0.9424 CYP450 inhibitory promiscuity Low CYP Inhibitory Promiscuity 0.8362 Ames test AMES toxic 0.5146 Carcinogenicity Non-carcinogens 0.5931 Biodegradation Not ready biodegradable 0.7893 Rat acute toxicity 2.9481 LD50, mol/kg Not applicable hERG inhibition (predictor I) Weak inhibitor 0.952 hERG inhibition (predictor II) Non-inhibitor 0.8738
Spectra
- Mass Spec (NIST)
- Not Available
- Spectra
Spectrum Spectrum Type Splash Key Predicted GC-MS Spectrum - GC-MS Predicted GC-MS Not Available Predicted MS/MS Spectrum - 10V, Positive (Annotated) Predicted LC-MS/MS Not Available Predicted MS/MS Spectrum - 20V, Positive (Annotated) Predicted LC-MS/MS Not Available Predicted MS/MS Spectrum - 40V, Positive (Annotated) Predicted LC-MS/MS Not Available LC-MS/MS Spectrum - LC-ESI-QQ , positive LC-MS/MS splash10-00di-0090000000-7feec7180feed2165741 LC-MS/MS Spectrum - LC-ESI-QQ , positive LC-MS/MS splash10-00di-0090000000-33ad4a0e0807f82bab39 LC-MS/MS Spectrum - LC-ESI-QQ , positive LC-MS/MS splash10-0ab9-2090000000-285ee2bce4f4c2097510 LC-MS/MS Spectrum - LC-ESI-QQ , positive LC-MS/MS splash10-00di-9110000000-a2386202e9dc8c7c753f LC-MS/MS Spectrum - LC-ESI-QQ , positive LC-MS/MS splash10-00di-9100000000-b80120a3f55bb762fe51 LC-MS/MS Spectrum - LC-ESI-IT , positive LC-MS/MS splash10-0a4i-0090000000-675c3ee4663579ce2125
Targets
- Kind
- Protein
- Organism
- Humans
- Pharmacological action
- Yes
- Actions
- Inhibitor
- General Function
- Serine hydrolase activity
- Specific Function
- Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
- Gene Name
- ACHE
- Uniprot ID
- P22303
- Uniprot Name
- Acetylcholinesterase
- Molecular Weight
- 67795.525 Da
References
- Trevisani GT, Hyman NH, Church JM: Neostigmine: safe and effective treatment for acute colonic pseudo-obstruction. Dis Colon Rectum. 2000 May;43(5):599-603. [PubMed:10826417]
- Naves LA, Van der Kloot W: Repetitive nerve stimulation decreases the acetylcholine content of quanta at the frog neuromuscular junction. J Physiol. 2001 May 1;532(Pt 3):637-47. [PubMed:11313435]
- Takeuchi K, Kawauchi S, Araki H, Ueki S, Furukawa O: Stimulation by nizatidine, a histamine H(2)-receptor antagonist, of duodenal HCO(3)(-)secretion in rats:relation to anti-cholinesterase activity. World J Gastroenterol. 2000 Oct;6(5):651-658. [PubMed:11819669]
- Minic J, Chatonnet A, Krejci E, Molgo J: Butyrylcholinesterase and acetylcholinesterase activity and quantal transmitter release at normal and acetylcholinesterase knockout mouse neuromuscular junctions. Br J Pharmacol. 2003 Jan;138(1):177-87. [PubMed:12522088]
- Beck KD, Brennan FX, Moldow RL, Ottenweller JE, Zhu G, Servatius RJ: Stress interacts with peripheral cholinesterase inhibitors to cause central nervous system effects. Life Sci. 2003 May 23;73(1):41-51. [PubMed:12726885]
- Zhang B, Hepner DL, Tran MH, Friedman M, Korn JR, Menzin J: Neuromuscular blockade, reversal agent use, and operating room time: retrospective analysis of US inpatient surgeries. Curr Med Res Opin. 2009 Apr;25(4):943-50. doi: 10.1185/03007990902769054 . [PubMed:19257799]
- Chen X, Ji ZL, Chen YZ: TTD: Therapeutic Target Database. Nucleic Acids Res. 2002 Jan 1;30(1):412-5. [PubMed:11752352]
Enzymes
- Kind
- Protein
- Organism
- Humans
- Pharmacological action
- Unknown
- Actions
- Inhibitor
- General Function
- Identical protein binding
- Specific Function
- Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
- Gene Name
- BCHE
- Uniprot ID
- P06276
- Uniprot Name
- Cholinesterase
- Molecular Weight
- 68417.575 Da
References
- Saito S: Cholinesterase inhibitors induce growth cone collapse and inhibit neurite extension in primary cultured chick neurons. Neurotoxicol Teratol. 1998 Jul-Aug;20(4):411-9. [PubMed:9697967]
Drug created on July 08, 2007 11:06 / Updated on September 22, 2020 02:10
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