Muscarinic acetylcholine receptor M3

Details

Name
Muscarinic acetylcholine receptor M3
Synonyms
Not Available
Gene Name
CHRM3
UniProtKB Entry
P20309Swiss-Prot
Organism
Humans
NCBI Taxonomy ID
9606
Amino acid sequence
>lcl|BSEQ0009898|Muscarinic acetylcholine receptor M3
MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFSSPDGTTDDPL
GGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVI
SMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKR
TTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAF
YMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAETENFVHPTGSSRSCSSYELQQQSM
KRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSE
TRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVDLERKADKLQAQKSVDDGGSF
PKSFSKLPIQLESAVDTAKTSDVNSSVGKSTATLPLSFKEATLAKRFALKTRSQITKRKR
MSLVKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYINSTVN
PVCYALCNKTFRTTFKMLLLCQCDKKKRRKQQYQQRQSVIFHKRAPEQAL
Number of residues
590
Molecular Weight
66127.445
Theoretical pI
9.62
GO Classification
Functions
G protein-coupled acetylcholine receptor activity / G protein-coupled serotonin receptor activity / signaling receptor activity
Processes
acetylcholine receptor signaling pathway / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / calcium-mediated signaling / chemical synaptic transmission / G protein-coupled acetylcholine receptor signaling pathway / G protein-coupled receptor signaling pathway / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / ion channel modulating, G protein-coupled receptor signaling pathway / ligand-gated ion channel signaling pathway / phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway / positive regulation of insulin secretion / protein modification process / regulation of monoatomic ion transmembrane transporter activity / regulation of smooth muscle contraction
Components
basal plasma membrane / endoplasmic reticulum membrane
General Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover
Specific Function
Acetylcholine binding
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
68-91 105-130 143-164 185-206 230-252 492-514 527-546
Cellular Location
Cell membrane
Gene sequence
>lcl|BSEQ0009899|Muscarinic acetylcholine receptor M3 (CHRM3)
ATGACCTTGCACAATAACAGTACAACCTCGCCTTTGTTTCCAAACATCAGCTCCTCCTGG
ATACACAGCCCCTCCGATGCAGGGCTGCCCCCGGGAACCGTCACTCATTTCGGCAGCTAC
AATGTTTCTCGAGCAGCTGGCAATTTCTCCTCTCCAGACGGTACCACCGATGACCCTCTG
GGAGGTCATACCGTCTGGCAAGTGGTCTTCATCGCTTTCTTAACGGGCATCCTGGCCTTG
GTGACCATCATCGGCAACATCCTGGTAATTGTGTCATTTAAGGTCAACAAGCAGCTGAAG
ACGGTCAACAACTACTTCCTCTTAAGCCTGGCCTGTGCCGATCTGATTATCGGGGTCATT
TCAATGAATCTGTTTACGACCTACATCATCATGAATCGATGGGCCTTAGGGAACTTGGCC
TGTGACCTCTGGCTTGCCATTGACTACGTAGCCAGCAATGCCTCTGTTATGAATCTTCTG
GTCATCAGCTTTGACAGATACTTTTCCATCACGAGGCCGCTCACGTACCGAGCCAAACGA
ACAACAAAGAGAGCCGGTGTGATGATCGGTCTGGCTTGGGTCATCTCCTTTGTCCTTTGG
GCTCCTGCCATCTTGTTCTGGCAATACTTTGTTGGAAAGAGAACTGTGCCTCCGGGAGAG
TGCTTCATTCAGTTCCTCAGTGAGCCCACCATTACTTTTGGCACAGCCATCGCTGCTTTT
TATATGCCTGTCACCATTATGACTATTTTATACTGGAGGATCTATAAGGAAACTGAAAAG
CGTACCAAAGAGCTTGCTGGCCTGCAAGCCTCTGGGACAGAGGCAGAGACAGAAAACTTT
GTCCACCCCACGGGCAGTTCTCGAAGCTGCAGCAGTTACGAACTTCAACAGCAAAGCATG
AAACGCTCCAACAGGAGGAAGTATGGCCGCTGCCACTTCTGGTTCACAACCAAGAGCTGG
AAACCCAGCTCCGAGCAGATGGACCAAGACCACAGCAGCAGTGACAGTTGGAACAACAAT
GATGCTGCTGCCTCCCTGGAGAACTCCGCCTCCTCCGACGAGGAGGACATTGGCTCCGAG
ACGAGAGCCATCTACTCCATCGTGCTCAAGCTTCCGGGTCACAGCACCATCCTCAACTCC
ACCAAGTTACCCTCATCGGACAACCTGCAGGTGCCTGAGGAGGAGCTGGGGATGGTGGAC
TTGGAGAGGAAAGCCGACAAGCTGCAGGCCCAGAAGAGCGTGGACGATGGAGGCAGTTTT
CCAAAAAGCTTCTCCAAGCTTCCCATCCAGCTAGAGTCAGCCGTGGACACAGCTAAGACT
TCTGACGTCAACTCCTCAGTGGGTAAGAGCACGGCCACTCTACCTCTGTCCTTCAAGGAA
GCCACTCTGGCCAAGAGGTTTGCTCTGAAGACCAGAAGTCAGATCACTAAGCGGAAAAGG
ATGTCCCTGGTCAAGGAGAAGAAAGCGGCCCAGACCCTCAGTGCGATCTTGCTTGCCTTC
ATCATCACTTGGACCCCATACAACATCATGGTTCTGGTGAACACCTTTTGTGACAGCTGC
ATACCCAAAACCTTTTGGAATCTGGGCTACTGGCTGTGCTACATCAACAGCACCGTGAAC
CCCGTGTGCTATGCTCTGTGCAACAAAACATTCAGAACCACTTTCAAGATGCTGCTGCTG
TGCCAGTGTGACAAAAAAAAGAGGCGCAAGCAGCAGTACCAGCAGAGACAGTCGGTCATT
TTTCACAAGCGCGCACCCGAGCAGGCCTTGTAG
Chromosome Location
1
Locus
1q43
External Identifiers
ResourceLink
UniProtKB IDP20309
UniProtKB Entry NameACM3_HUMAN
GenBank Protein ID32324
GenBank Gene IDX15266
GeneCard IDCHRM3
GenAtlas IDCHRM3
HGNC IDHGNC:1952
PDB ID(s)2CSA, 8E9W, 8E9Y, 8E9Z, 8EA0
KEGG IDhsa:1131
IUPHAR/Guide To Pharmacology ID15
NCBI Gene ID1131
General References
  1. Peralta EG, Ashkenazi A, Winslow JW, Smith DH, Ramachandran J, Capon DJ: Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors. EMBO J. 1987 Dec 20;6(13):3923-9. [Article]
  2. Bonner TI, Young AC, Brann MR, Buckley NJ: Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes. Neuron. 1988 Jul;1(5):403-10. [Article]
  3. Kitano T, Liu YH, Ueda S, Saitou N: Human-specific amino acid changes found in 103 protein-coding genes. Mol Biol Evol. 2004 May;21(5):936-44. Epub 2004 Mar 10. [Article]
  4. Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke G, Clee C, Cobley V, Collier RE, Corby N, Coville GJ, Davies J, Deadman R, Dunn M, Earthrowl M, Ellington AG, Errington H, Frankish A, Frankland J, French L, Garner P, Garnett J, Gay L, Ghori MR, Gibson R, Gilby LM, Gillett W, Glithero RJ, Grafham DV, Griffiths C, Griffiths-Jones S, Grocock R, Hammond S, Harrison ES, Hart E, Haugen E, Heath PD, Holmes S, Holt K, Howden PJ, Hunt AR, Hunt SE, Hunter G, Isherwood J, James R, Johnson C, Johnson D, Joy A, Kay M, Kershaw JK, Kibukawa M, Kimberley AM, King A, Knights AJ, Lad H, Laird G, Lawlor S, Leongamornlert DA, Lloyd DM, Loveland J, Lovell J, Lush MJ, Lyne R, Martin S, Mashreghi-Mohammadi M, Matthews L, Matthews NS, McLaren S, Milne S, Mistry S, Moore MJ, Nickerson T, O'Dell CN, Oliver K, Palmeiri A, Palmer SA, Parker A, Patel D, Pearce AV, Peck AI, Pelan S, Phelps K, Phillimore BJ, Plumb R, Rajan J, Raymond C, Rouse G, Saenphimmachak C, Sehra HK, Sheridan E, Shownkeen R, Sims S, Skuce CD, Smith M, Steward C, Subramanian S, Sycamore N, Tracey A, Tromans A, Van Helmond Z, Wall M, Wallis JM, White S, Whitehead SL, Wilkinson JE, Willey DL, Williams H, Wilming L, Wray PW, Wu Z, Coulson A, Vaudin M, Sulston JE, Durbin R, Hubbard T, Wooster R, Dunham I, Carter NP, McVean G, Ross MT, Harrow J, Olson MV, Beck S, Rogers J, Bentley DR, Banerjee R, Bryant SP, Burford DC, Burrill WD, Clegg SM, Dhami P, Dovey O, Faulkner LM, Gribble SM, Langford CF, Pandian RD, Porter KM, Prigmore E: The DNA sequence and biological annotation of human chromosome 1. Nature. 2006 May 18;441(7091):315-21. [Article]
  5. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
  6. Speek M: Antisense promoter of human L1 retrotransposon drives transcription of adjacent cellular genes. Mol Cell Biol. 2001 Mar;21(6):1973-85. [Article]
  7. Yang J, Williams JA, Yule DI, Logsdon CD: Mutation of carboxyl-terminal threonine residues in human m3 muscarinic acetylcholine receptor modulates the extent of sequestration and desensitization. Mol Pharmacol. 1995 Sep;48(3):477-85. [Article]
  8. Weber S, Thiele H, Mir S, Toliat MR, Sozeri B, Reutter H, Draaken M, Ludwig M, Altmuller J, Frommolt P, Stuart HM, Ranjzad P, Hanley NA, Jennings R, Newman WG, Wilcox DT, Thiel U, Schlingmann KP, Beetz R, Hoyer PF, Konrad M, Schaefer F, Nurnberg P, Woolf AS: Muscarinic Acetylcholine Receptor M3 Mutation Causes Urinary Bladder Disease and a Prune-Belly-like Syndrome. Am J Hum Genet. 2011 Nov 11;89(5):668-74. doi: 10.1016/j.ajhg.2011.10.007. [Article]
  9. Patowary S, Alvarez-Curto E, Xu TR, Holz JD, Oliver JA, Milligan G, Raicu V: The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane. Biochem J. 2013 Jun 1;452(2):303-12. doi: 10.1042/BJ20121902. [Article]
  10. Iverson HA, Fox D 3rd, Nadler LS, Klevit RE, Nathanson NM: Identification and structural determination of the M(3) muscarinic acetylcholine receptor basolateral sorting signal. J Biol Chem. 2005 Jul 1;280(26):24568-75. Epub 2005 May 2. [Article]

Associated Data

Bio-Entities
Bio-EntityType
Muscarinic acetylcholine receptor M3 (Humans)protein
primary
Muscarinic acetylcholine receptor (Humans)protein
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Darifenacinapproved, investigationalyestargetantagonistDetails
Diphemanilapproved, vet_approved, withdrawnyestargetantagonistDetails
CevimelineapprovedyestargetagonistDetails
Olanzapineapproved, investigationalunknowntargetantagonistDetails
OxyphencyclimineapprovedyestargetantagonistDetails
TridihexethylwithdrawnyestargetantagonistDetails
Anisotropine methylbromideapprovedyestargetantagonistDetails
Atropineapproved, vet_approvedyestargetantagonistDetails
Homatropine methylbromideapprovedyestargetantagonistDetails
BenzquinamidewithdrawnyestargetantagonistDetails
Promethazineapproved, investigationalunknowntargetantagonistDetails
Diphenidolapproved, investigational, withdrawnyestargetantagonistDetails
Methotrimeprazineapproved, investigationalunknowntargetantagonistDetails
TiotropiumapprovedyestargetantagonistDetails
SolifenacinapprovedyestargetantagonistDetails
Oxybutyninapproved, investigationalyestargetantagonistDetails
Tolterodineapproved, investigationalyestargetantagonistDetails
ALKS 27investigationalunknowntargetDetails
Ipratropiumapproved, experimentalyestargetantagonistDetails
Nicardipineapproved, investigationalunknowntargetantagonistDetails
FesoterodineapprovedyestargetantagonistDetails
QuetiapineapprovedunknowntargetantagonistDetails
ZiprasidoneapprovedunknowntargetantagonistDetails
ClozapineapprovedunknowntargetantagonistDetails
PipecuroniumapprovedunknowntargetantagonistDetails
PancuroniumapprovedunknowntargetantagonistDetails
DisopyramideapprovedunknowntargetantagonistDetails
Terfenadineapproved, withdrawnunknowntargetantagonistDetails
HyoscyamineapprovedunknowntargetantagonistDetails
Chlorprothixeneapproved, experimental, investigational, withdrawnnotargetantagonistDetails
Doxepinapproved, investigationalunknowntargetantagonistDetails
Desipramineapproved, investigationalnotargetantagonistDetails
ImipramineapprovednotargetantagonistDetails
CyproheptadineapprovedunknowntargetantagonistDetails
BrompheniramineapprovedunknowntargetantagonistDetails
Tropicamideapproved, investigationalunknowntargetantagonistDetails
Scopolamineapproved, investigationalyestargetantagonistDetails
ProcyclidineapprovedyestargetantagonistDetails
TrihexyphenidylapprovedunknowntargetantagonistDetails
MivacuriumapprovednotargetantagonistDetails
Pilocarpineapproved, investigationalyestargetagonistDetails
SuccinylcholineapprovedunknowntargetagonistDetails
Maprotilineapproved, investigationalnotargetantagonistDetails
MepenzolateapprovedyestargetantagonistDetails
Isopropamideapproved, vet_approvedyestargetantagonistDetails
Methacholineapproved, investigationalyestargetagonistDetails
Tramadolapproved, investigationalunknowntargetantagonistDetails
Methscopolamine bromideapprovedunknowntargetantagonistDetails
AclidiniumapprovedyestargetantagonistDetails
Chlorpromazineapproved, investigational, vet_approvedunknowntargetantagonistDetails
LoxapineapprovedunknowntargetbinderDetails
Glycopyrroniumapproved, investigational, vet_approvedyestargetantagonistDetails
UmeclidiniumapprovedyestargetantagonistDetails
HexocycliumapprovedyestargetantagonistDetails
Butylscopolamineapproved, investigational, vet_approvedyestargetantagonistDetails
DosulepinapprovedyestargetantagonistDetails
ImidafenacininvestigationalyestargetantagonistDetails
Acetylcholineapproved, investigationalunknowntargetDetails
Thiopentalapproved, vet_approvedunknowntargetDetails
CarbamoylcholineapprovedunknowntargetagonistDetails
ArecolineexperimentalunknowntargetDetails
BethanecholapprovedyestargetagonistDetails
HomatropineapprovedyestargetantagonistDetails
PizotifenapprovedunknowntargetantagonistDetails
Propiverineapproved, investigationalyestargetantagonistDetails
ThonzylamineapprovedunknowntargetantagonistDetails
RociverineexperimentalyestargetantagonistDetails
TrimebutineapprovedyestargetantagonistDetails
Aripiprazole lauroxilapproved, investigationalnotargetDetails
HaloperidolapprovedunknowntargetDetails
Aripiprazoleapproved, investigationalunknowntargetligandDetails
TrospiumapprovedunknowntargetDetails
Methanthelineapproved, investigationalunknowntargetDetails
DicyclomineapprovedunknowntargetantagonistDetails
Viloxazineapproved, investigational, withdrawnunknowntargetantagonistDetails
Sofpironiumapproved, investigationalyestargetinhibitorDetails
Vinburnineexperimentalyestargetallosteric modulatorDetails
TarafenacininvestigationalyestargetantagonistDetails
MethscopolamineapprovedyestargetantagonistDetails
AceclidineapprovedyestargetinhibitorDetails
MetixeneapprovedyestargetantagonistDetails
Doxylamineapproved, vet_approvedunknowntargetantagonistDetails
PropiomazineapprovedunknowntargetantagonistDetails
AmoxapineapprovedunknowntargetantagonistDetails
TrimipramineapprovedunknowntargetbinderDetails
MeperidineapprovedunknowntargetbinderDetails
Cinnarizineapproved, investigationalunknowntargetbinderDetails
Ketamineapproved, vet_approvedunknowntargetbinderDetails
EtoperidonewithdrawnunknowntargetantagonistDetails
Revefenacinapproved, investigationalyestargetantagonistDetails
AmitriptylineapprovedunknowntargetligandDetails
Paroxetineapproved, investigationalunknowntargetinhibitorDetails
NortriptylineapprovedunknowntargetantagonistDetails
Sofpironiumapproved, investigationalyestargetinhibitorDetails